LOCUS       sequence-129514-       13943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline-inducible lentiviral expression of human ARID1A; 3rd 
            generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Guan B, Wang TL, Shih IeM
  TITLE     ARID1A, a factor that promotes formation of SWI/SNF-mediated 
            chromatin remodeling, is a tumor suppressor in gynecologic cancers.
  JOURNAL   Cancer Res. 2011 Nov 1;71(21):6718-27. Epub 2011 Sep 7.
  PUBMED    21900401
REFERENCE   2  (bases 1 to 13943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Res."; date: "2011-11-1"; volume: "71(21)"; pages: "6718-27. Epub 
            2011 Sep 7"
COMMENT     Sequence Label: pLenti-puro-ARID1A
FEATURES             Location/Qualifiers
     source          1..13943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2404..2607
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2558..2578
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2609..2627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2630..2648
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      2726..2735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             5512..5520
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9614..9655
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9665..9682
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9705..9722)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(9734..9754)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9739..10068
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9919..10054
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10078..10677
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10078..10097)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10574..10594
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10749..10982
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11054..11175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11091..11110)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11145..11164
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11215..11350
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11370..11389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11371..11389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11399..11416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11399..11415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11408..11430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11557..12012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11644..11663)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11854..11875
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12038..12142
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12143..12211,12212..13003)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12361..12380)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13174..13762
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13663..13682
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13916..13933
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2041 agacagatcc attcgattag tgaacggatc tcgacggtat cgataagctt gggagttccg
     2101 cttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2161 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2221 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2281 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2341 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2401 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2461 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2521 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2581 cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct ccctatcagt
     2641 gatagagatc gtcgactagt ggatccagtg tggtggaatt ctgcagatat ccagcacagt
     2701 ggcggccgct cgagtctagc ctcgagccac catggccgcg caggtcgccc ccgccgccgc
     2761 cagcagcctg ggcaacccgc cgccgccgcc gccctcggag ctgaagaaag ccgagcagca
     2821 gcagcgggag gaggcggggg gcgaggcggc ggcggcggca gcggccgagc gcggggaaat
     2881 gaaggcagcc gccgggcagg aaagcgaggg ccccgccgtg gggccgccgc agccgctggg
     2941 aaaggagctg caggacgggg ccgagagcaa tgggggtggc ggcggcggcg gagccggcag
     3001 cggcggcggg cccggcgcgg agccggacct gaagaactcg aacgggaacg cgggccctag
     3061 gcccgccctg aacaataacc tcacggagcc gcccggcggc ggcggtggcg gcagcagcga
     3121 tggggtgggg gcgcctcctc actcagccgc ggccgccttg ccgcccccag cctacggctt
     3181 cgggcaaccc tacggccgga gcccgtctgc cgtcgccgcc gccgcggccg ccgtcttcca
     3241 ccaacaacat ggcggacaac aaagccctgg cctggcagcg ctgcagagcg gcggcggcgg
     3301 gggcctggag ccctacgcgg ggccccagca gaactctcac gaccacggct tccccaacca
     3361 ccagtacaac tcctactacc ccaaccgcag cgcctacccc ccgcccgccc cggcctacgc
     3421 gctgagctcc ccgagaggtg gcactccggg ctccggcgcg gcggcggctg ccggctccaa
     3481 gccgcctccc tcctccagcg cctccgcctc ctcgtcgtct tcgtccttcg ctcagcagcg
     3541 cttcggggcc atggggggag gcggcccctc cgcggccggc gggggaactc cccagcccac
     3601 cgccaccccc accctcaacc aactgctcac gtcgcccagc tcggcccggg gctaccaggg
     3661 ctaccccggg ggcgactaca gtggcgggcc ccaggacggg ggcgccggca agggcccggc
     3721 ggacatggcc tcgcagtgtt ggggggctgc ggcggcggca gctgcggcgg cggccgcctc
     3781 gggaggggcc caacaaagga gccaccacgc gcccatgagc cccgggagca gcggcggcgg
     3841 ggggcagccg ctcgcccgga cccctcagcc atccagtcca atggatcaga tgggcaagat
     3901 gagacctcag ccatatggcg ggactaaccc atactcgcag caacagggac ctccgtcagg
     3961 accgcagcaa ggacatgggt acccagggca gccatacggg tcccagaccc cgcagcggta
     4021 cccgatgacc atgcagggcc gggcgcagag tgccatgggc ggcctctctt atacacagca
     4081 gattcctcct tatggacaac aaggccccag cgggtatggt caacagggcc agactccata
     4141 ttacaaccag caaagtcctc accctcagca gcagcagcca ccctactccc agcaaccacc
     4201 gtcccagacc cctcatgccc aaccttcgta tcagcagcag ccacagtctc aaccaccaca
     4261 gctccagtcc tctcagcctc catactccca gcagccatcc cagcctccac atcagcagtc
     4321 cccggctcca tacccctccc agcagtcgac gacacagcag cacccccaga gccagccccc
     4381 ctactcacag ccacaggctc agtctcctta ccagcagcag caacctcagc agccagcacc
     4441 ctcgacgctc tcccagcagg ctgcgtatcc tcagccccag tctcagcagt cccagcaaac
     4501 tgcctattcc cagcagcgct tccctccacc gcaggagcta tctcaagatt catttgggtc
     4561 tcaggcatcc tcagccccct caatgacctc cagtaaggga gggcaagaag atatgaacct
     4621 gagccttcag tcaagaccct ccagcttgcc tgatctatct ggttcaatag atgacctccc
     4681 catggggaca gaaggagctc tgagtcctgg agtgagcaca tcagggattt ccagcagcca
     4741 aggagagcag agtaatccag ctcagtctcc tttctctcct catacctccc ctcacctgcc
     4801 tggcatccga ggcccttccc cgtcccctgt tggctctccc gccagtgttg ctcagtctcg
     4861 ctcaggacca ctctcgcctg ctgcagtgcc aggcaaccag atgccacctc ggccacccag
     4921 tggccagtcg gacagcatca tgcatccttc catgaaccaa tcaagcattg cccaagatcg
     4981 aggttatatg cagaggaacc cccagatgcc ccagtacagt tccccccagc ccggctcagc
     5041 cttatctccg cgtcagcctt ccggaggaca gatacacaca ggcatgggct cctaccagca
     5101 gaactccatg gggagctatg gtccccaggg gggtcagtat ggcccacaag gtggctaccc
     5161 caggcagcca aactataatg ccttgcccaa tgccaactac cccagtgcag gcatggctgg
     5221 aggcataaac cccatgggtg ccggaggtca aatgcatgga cagcctggca tcccacctta
     5281 tggcacactc cctccaggga ggatgagtca cgcctccatg ggcaaccggc cttatggccc
     5341 taacatggcc aatatgccac ctcaggttgg gtcagggatg tgtcccccac cagggggcat
     5401 gaaccggaaa acccaagaaa ctgctgtcgc catgcatgtt gctgccaact ctatccaaaa
     5461 caggccgcca ggctacccca atatgaatca agggggcatg atgggaactg gacctcctta
     5521 tggacaaggg attaatagta tggctggcat gatcaaccct cagggacccc catattccat
     5581 gggtggaacc atggccaaca attctgcagg gatggcagcc agcccagaga tgatgggcct
     5641 tggggatgta aagttaactc cagccaccaa aatgaacaac aaggcagatg ggacacccaa
     5701 gacagaatcc aaatccaaga aatccagttc ttctactaca accaatgaga agatcaccaa
     5761 gttgtatgag ctgggtggtg agcctgagag gaagatgtgg gtggaccgtt atctggcctt
     5821 cactgaggag aaggccatgg gcatgacaaa tctgcctgct gtgggtagga aacctctgga
     5881 cctctatcgc ctctatgtgt ctgtgaagga gattggtgga ttgactcagg tcaacaagaa
     5941 caaaaaatgg cgggaacttg caaccaacct caatgtgggc acatcaagca gtgctgccag
     6001 ctccttgaaa aagcagtata tccagtgtct ctatgccttt gaatgcaaga ttgaacgggg
     6061 agaagaccct cccccagaca tctttgcagc tgctgattcc aagaagtccc agcccaagat
     6121 ccagcctccc tctcctgcgg gatcaggatc tatgcagggg ccccagactc cccagtcaac
     6181 cagcagttcc atggcagaag gaggagactt aaagccacca actccagcat ccacaccaca
     6241 cagtcagatc cccccattgc caggcatgag caggagcaat tcagttggga tccaggatgc
     6301 ctttaatgat ggaagtgact ccacattcca gaagcggaat tccatgactc caaaccctgg
     6361 gtatcagccc agtatgaata cctctgacat gatggggcgc atgtcctatg agccaaataa
     6421 ggatccttat ggcagcatga ggaaagctcc agggagtgat cccttcatgt cctcagggca
     6481 gggccccaac ggcgggatgg gtgaccccta cagtcgtgct gccggccctg ggctaggaaa
     6541 tgtggcgatg ggaccacgac agcactatcc ctatggaggt ccttatgaca gagtgaggac
     6601 ggagcctgga atagggcctg agggaaacat gagcactggg gccccacagc cgaatctcat
     6661 gccttccaac ccagactcgg ggatgtattc tcctagccgc taccccccgc agcagcagca
     6721 gcagcagcag caacgacatg attcctatgg caatcagttc tccacccaag gcaccccttc
     6781 tggcagcccc ttccccagcc agcagactac aatgtatcaa cagcaacagc agaattacaa
     6841 gcggccaatg gatggcacat atggccctcc tgccaagcgg cacgaagggg agatgtacag
     6901 cgtgccatac agcactgggc aggggcagcc tcagcagcag cagttgcccc cagcccagcc
     6961 ccagcctgcc agccagcaac aagctgccca gccttcccct cagcaagatg tatacaacca
     7021 gtatggcaat gcctatcctg ccactgccac agctgctact gagcgccgac cagcaggcgg
     7081 cccccagaac caatttccat tccagtttgg ccgagaccgt gtctctgcac cccctggcac
     7141 caatgcccag caaaacatgc caccacaaat gatgggcggc cccatacagg catcagctga
     7201 ggttgctcag caaggcacca tgtggcaggg gcgtaatgac atgacctata attatgccaa
     7261 caggcagagc acgggctctg ccccccaggg ccccgcctat catggcgtga accgaacaga
     7321 tgaaatgctg cacacagatc agagggccaa ccacgaaggc tcgtggcctt cccatggcac
     7381 acgccagccc ccatatggtc cctctgcccc tgtgcccccc atgacaaggc cccctccatc
     7441 taactaccag cccccaccaa gcatgcagaa tcacattcct caggtatcca gccctgctcc
     7501 cctgccccgg ccaatggaga accgcacctc tcctagcaag tctccattcc tgcactctgg
     7561 gatgaaaatg cagaaggcag gtcccccagt acctgcctcg cacatagcac ctgcccctgt
     7621 gcagcccccc atgattcggc gggatatcac cttcccacct ggctctgttg aagccacaca
     7681 gcctgtgttg aagcagagga ggcggctcac aatgaaagac attggaaccc cggaggcatg
     7741 gcgggtaatg atgtccctca agtctggtct cctggcagag agcacatggg cattagatac
     7801 catcaacatc ctgctgtatg atgacaacag catcatgacc ttcaacctca gtcagctccc
     7861 agggttgcta gagctccttg tagaatattt ccgacgatgc ctgattgaga tctttggcat
     7921 tttaaaggag tatgaggtgg gtgacccagg acagagaacg ctactggatc ctgggaggtt
     7981 cagcaaggtg tctagtccag ctcccatgga gggtggggaa gaagaagaag aacttctagg
     8041 tcctaaacta gaagaggaag aagaagagga agtagttgaa aatgatgagg agatagcctt
     8101 ttcaggcaag gacaagccag cttcagagaa tagtgaggag aagctgatca gtaagtttga
     8161 caagcttcca gtaaagatcg tacagaagaa tgatccattt gtggtggact gctcagataa
     8221 gcttgggcgt gtgcaggagt ttgacagtgg cctgctgcac tggcggattg gtggggggga
     8281 caccactgag catatccaga cccacttcga gagcaagaca gagctgctgc cttcccggcc
     8341 tcacgcaccc tgcccaccag cccctcggaa gcatgtgaca acagcagagg gtacaccagg
     8401 gacaacagac caggaggggc ccccacctga tggacctcca gaaaaacgga tcacagccac
     8461 tatggatgac atgttgtcta ctcggtctag caccttgacc gaggatggag ctaagagttc
     8521 agaggccatc aaggagagca gcaagtttcc atttggcatt agcccagcac agagccaccg
     8581 gaacatcaag atcctagagg acgaacccca cagtaaggat gagaccccac tgtgtaccct
     8641 tctggactgg caggattctc ttgccaagcg ctgcgtctgt gtgtccaata ccattcgaag
     8701 cctgtcattt gtgccaggca atgactttga gatgtccaaa cacccagggc tgctgctcat
     8761 cctgggcaag ctgatcctgc tgcaccacaa gcacccagaa cggaagcagg caccactaac
     8821 ttatgaaaag gaggaggaac aggaccaagg ggtgagctgc aacaaagtgg agtggtggtg
     8881 ggactgcttg gagatgctcc gggaaaacac cttggttaca ctcgccaaca tctcggggca
     8941 gttggaccta tctccatacc ccgagagcat ttgcctgcct gtcctggacg gactcctaca
     9001 ctgggcagtt tgcccttcag ctgaagccca ggaccccttt tccaccctgg gccccaatgc
     9061 cgtcctttcc ccgcagagac tggtcttgga aaccctcagc aaactcagca tccaggacaa
     9121 caatgtggac ctgattctgg ccacaccccc cttcagccgc ctggagaagt tgtatagcac
     9181 tatggtgcgc ttcctcagtg accgaaagaa cccggtgtgc cgggagatgg ctgtggtact
     9241 gctggccaac ctggctcagg gggacagcct ggcagctcgt gccattgcag tgcagaaggg
     9301 cagtatcggc aacctcctgg gcttcctaga ggacagcctt gccgccacac agttccagca
     9361 gagccaggcc agcctcctcc acatgcagaa cccacccttt gagccaacta gtgtggacat
     9421 gatgcggcgg gctgcccgcg cgctgcttgc cttggccaag gtggacgaga accactcaga
     9481 gtttactctg tacgaatcac ggctgttgga catctcggta tcaccgttga tgaactcatt
     9541 ggtttcacaa gtcatttgtg atgtactgtt tttgattggc cagtcctcga gtctagaggg
     9601 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     9661 cggtcatcat caccatcacc attgagttta aacctgctga tcagcctcga ctgtgccttc
     9721 tagtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9841 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9901 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9961 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    10021 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttaccatg
    10081 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
    10141 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
    10201 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
    10261 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
    10321 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
    10381 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
    10441 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
    10501 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
    10561 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
    10621 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaggt
    10681 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
    10741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
    10801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
    10861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
    10921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
    10981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
    11041 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11101 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11161 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
    11221 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    11281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    11341 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
    11401 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    11461 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    11521 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
    11581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
    11641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
    11701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
    11761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
    11821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
    11881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
    11941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
    12001 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    12061 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    12121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    12181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    12241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    12301 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    12361 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    12421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    12481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    12541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    12601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    12661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    12721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    12781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    12841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    12901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    12961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    13021 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    13081 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    13141 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    13201 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    13261 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    13321 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    13381 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    13441 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    13501 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    13561 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    13621 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    13681 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    13741 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    13801 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    13861 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    13921 gtcagtgagc gaggaagcgg aag
//
