LOCUS       sequence-129515-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constiutive mammalian expression of the bioluminescent GpNLuc 
            LumiFluor reporter for single-cell analysis and sensitive optical 
            imaging of living subjects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, 
            Cleveland JL, Amelio AL
  TITLE     Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical 
            Imaging and Flow Cytometry for Monitoring Tumorigenesis.
  JOURNAL   Cancer Res. 2015 Sep 30. pii: canres.3538.2014.
  PUBMED    26424696
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Sep 30. pii: canres.3538.2014."
COMMENT     Sequence Label: pRetroX-Tight-MCS_PGK-GpNLuc
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(65..580)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(638..659)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtactctata ggcttcagct ggtgatattg ttgagtcaaa actagagcct ggaccactat
       61 cgatttacgc cagaatgcgt tcgcacagcc gccagccggt cactccgttg atggttactc
      121 ggaacagcag ggagccgtcg gggttgatca ggcgctcgtc gataattttg ttgccgttcc
      181 acagggtccc tgttacagtg atctttttgc cgtcgaacac ggcgatgcct tcatacggcc
      241 gtccgaaata gtcgatcatg ttcggcgtaa ccccgtcgat taccagtgtg ccatagtgca
      301 ggatcacctt aaagtgatga tcatccacag ggtacaccac cttaaaaatt ttttcgatct
      361 ggcccatttg gtcgccgctc agaccttcat acgggatgat gacatggatg tcgatcttca
      421 gcccattttc accgctcagg acaatccttt ggatcggagt tacggacacc ccgagattct
      481 gaaacaaact ggacacacct ccctgttcaa ggacttggtc caggttgtag ccggctgtct
      541 gtcgccagtc cccaacgaaa tcttcgagtg tgaagaccat acctccggag atatccttgt
      601 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      661 gatcgcgctt ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt
      721 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      781 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct
      841 gcttgtcggc catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcc
//
