LOCUS       sequence-129517-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease in mammalian cells with BFP tracker in 
            lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             65..91
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             95..115
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cccctcactc ggcgcgccag tcctccgaca gactgagtcg cccgctcgag ccgccaccat
       61 gggatatcca tacgatgtcc cagattatgc gccacctaag aagaaacgca aagtcgaatt
      121 caagaacatc aagaagaacc aggtcatgaa cctgggcccc aacagcaagc tgctgaagga
      181 gtacaagagc cagctgatcg agctgaacat cgagcagttc gaggccggca tcggcctgat
      241 cctgggcgac gcctacatca ggagcaggga cgagggcaag acctactgca tgcagttcga
      301 gtggaagaac aaggcctaca tggaccacgt gtgcctgctg tacgaccagt gggtgctgag
      361 ccccccccac aagaaggaga gggtgaacca cctgggcaac ctggtcatca cctggggcgc
      421 ccagaccttc aagcaccagg ccttcaacaa gctggccaac ctgttcatcg tgaacaacaa
      481 gaagaccatc cccaacaacc tggtggagaa ctacctgacc cccatgagcc tggcctactg
      541 gttcatggac gacggcggca agtgggacta caacaagaac agcaccaaca agagcatcgt
      601 gctgaacacc cagagcttca ccttcgagga ggtggagtac ctggtgaagg gcctgaggaa
      661 caagttccag ctgaactgct acgtgaagat caacaagaac aagcccatca tctacatcga
      721 cagcatgagc tacctgatct tctacaacct gatcaagccc tacctgatcc cccagatgat
      781 gtacaagctg cccaacacca tcagcagcga gaccttcctg aagtagcctg caggtcgagc
      841 atgcatctag ggcggccaat tccgcccct
//
