LOCUS       sequence-129518-        3063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLEX switch empty backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Atasoy D, Aponte Y, Su HH, Sternson SM
  TITLE     A FLEX switch targets Channelrhodopsin-2 to multiple cell types for 
            imaging and long-range circuit mapping.
  JOURNAL   J Neurosci. 2008 Jul 9. 28(28):7025-30.
  PUBMED    18614669
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2008 Jul 9. 28(28):7025-30."
COMMENT     Sequence Label: pJ241-FLEX
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..105
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     89..105
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    575..608
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    655..688
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    759..792
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(799..832)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(895..928)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(945..978)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(1185..1204)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        1271..1362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1363..2172
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2294..2882
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2783..2802
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3036..3053
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgagagtag ggaactgcca ggcatcaaac taagcagaag gcccctgacg gatggccttt
       61 ttgcgtttct acaaactctt tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc
      121 cctgggcggt tctgataacg agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg
      181 gcgggttttt ttatgggggg agtttaggga aagagcattt gtcagaatat ttaagggcgc
      241 ctgtcacttt gcttgatata tgagaattat ttaaccttat aaatgagaaa aaagcaacgc
      301 actttaaata agatacgttg ctttttcgat tgatgaacac ctataattaa actattcatc
      361 tattatttat gattttttgt atatacaata tttctagttt gttaaagaga attaagaaaa
      421 taaatctcga aaataataaa gggaaaatca gtttttgata tcaaaattat acatgtcaac
      481 gataatacaa aatataatac aaactataag atgttatcag tatttattat gcatttagaa
      541 taaattttgt gtcgccctta ttcgactcac tatagaagtt cctattctct agaaagtata
      601 ggaacttcac ttcattttct agagtttaaa cgaattcgaa cctcttcgag ggacataact
      661 tcgtataatg tatgctatac gaagttatgg gtcgatggtg agatctggac tagagggtcg
      721 atggtgatgc ttggatcaaa ataggaagaa tcccaatgat aacttcgtat aaagtatcct
      781 atacgaagtt atactagtat aacttcgtat agcatacatt atacgaagtt atgggtcgat
      841 ggtgatgctt ggcaattcgg gtcgatggtg aagcattggt cttcctattt tgatataact
      901 tcgtatagga tactttatac gaagttatgg ggatccccac ttcagaagtt cctatacttt
      961 ctagagaata ggaacttcac tatagagtcg aataagggcg acacccccta attagcccgg
     1021 gcgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta
     1081 ctctcgcatg gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg
     1141 gcatggggtc aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag
     1201 ccatattcag gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca
     1261 ctgaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1321 taaccctgat aaatgcttca ataatattga aaaaggaaga atatgagcca tattcaacgg
     1381 gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     1441 gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat
     1501 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     1561 atggtcagac taaactggct gacggaattt atgccacttc cgaccatcaa gcattttatc
     1621 cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcgttccag
     1681 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     1741 cgccggttgc actcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgc
     1801 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     1861 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     1921 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     1981 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     2041 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     2101 caaaaatatg gtattgataa tcctgatatg aataaattgc aatttcattt gatgctcgat
     2161 gagtttttct aagcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     2221 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2281 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2341 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2401 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2461 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2521 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2581 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2641 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2701 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2761 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2821 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2881 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2941 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3001 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3061 aag
//
