LOCUS       sequence-129520-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constiutive mammalian expression of the bioluminescent OgNLuc 
            LumiFluor reporter for single-cell analysis and sensitive optical 
            imaging of living subjects..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, 
            Cleveland JL, Amelio AL
  TITLE     Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical 
            Imaging and Flow Cytometry for Monitoring Tumorigenesis.
  JOURNAL   Cancer Res. 2015 Sep 30. pii: canres.3538.2014.
  PUBMED    26424696
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2015 Sep 30. pii: canres.3538.2014."
COMMENT     Sequence Label: pRetroX-Tight-MCS_PGK-OgNLuc
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(62..577)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 ctctataggc ttcagctggt gatattgttg agtcaaaact agagcctgga ccactatcga
       61 tttacgccag aatgcgttcg cacagccgcc agccggtcac tccgttgatg gttactcgga
      121 acagcaggga gccgtcgggg ttgatcaggc gctcgtcgat aattttgttg ccgttccaca
      181 gggtccctgt tacagtgatc tttttgccgt cgaacacggc gatgccttca tacggccgtc
      241 cgaaatagtc gatcatgttc ggcgtaaccc cgtcgattac cagtgtgcca tagtgcagga
      301 tcaccttaaa gtgatgatca tccacagggt acaccacctt aaaaattttt tcgatctggc
      361 ccatttggtc gccgctcaga ccttcatacg ggatgatgac atggatgtcg atcttcagcc
      421 cattttcacc gctcaggaca atcctttgga tcggagttac ggacaccccg agattctgaa
      481 acaaactgga cacacctccc tgttcaagga cttggtccag gttgtagccg gctgtctgtc
      541 gccagtcccc aacgaaatct tcgagtgtga agaccatacc tccggagata tccttgtaca
      601 gctcgtccat gccgccggtg gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg
      661 tgtagtcctc gttgtgggag gtgatgtcca acttgatgtc gacgatgtag gcgccgggca
      721 actgcacggg cttcttggcc ttgtaggtgg tcttgacctc ggaggtgtag tggccgccgt
      781 ccttcagctt cagcctgagc ttgtcctcgc ccttcagg
//
