LOCUS       sequence-129522-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VMA intein with C-terminal portion of alpha-sarcin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CSarcin
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atagggccct ctagatgcat gctcgagtta gtgagagcac agtttcagtt caccctggtt
       61 ttctttagtg tgagcgatga taccgcagaa aactttgttc gggtaagtgt agataacacg
      121 agccggaccc gggttttctt tcggtttttt agagtcgaat ttgtagtcgt gaccgtccgg
      181 gaaagtcggg aactccagca ggtagtggtc agttttaccg ttaccgtctt tagagtgttt
      241 cggcggacgg tcgcagtcag atttaccgaa ttttatcgga gtacgacctt tcggcagttt
      301 accgtcaccg tcgtaaccgt tagtgaacca gtgcgggtaa gaggagccag ttttaccgtc
      361 agacagcgga gcgtggtgag agttagattc agctttgcaa ttgtgcacga caacctggtt
      421 ggcaagcaaa aactgatgat cagaatcatc agataaagta atcccataat aatcgtcttc
      481 cttcaattct tgtaactcga aataaaatcc gcggcactca cgtgcaaaag cagcggcggg
      541 agcaggcctg aattttttag agccggcaca cttcgaaaga acgttaagca aaacactagt
      601 ctttgagatt cgtcggaaca catgataata gaggtcccag gcttggagga ggtccttgac
      661 attccctgat ttcatgtact tcctgcacca ctcttgggcc tccattaaat ctcgaccata
      721 ggcctgatta aaggatgttt ccttcagagt ctgggggccc cgttccatca tagcatgcaa
      781 gggctccagc acctcaaaca tgcctttcac gttcctttcc
//
