LOCUS       sequence-129523-        9577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9577)
  AUTHORS   Buganim Y, Faddah DA, Cheng AW, Itskovich E, Markoulaki S, Ganz K, 
            Klemm SL, van Oudenaarden A, Jaenisch R
  TITLE     Single-Cell Expression Analyses during Cellular Reprogramming Reveal
            an Early Stochastic and a Late Hierarchic Phase.
  JOURNAL   Cell. 2012 Sep 14;150(6):1209-22. doi: 10.1016/j.cell.2012.08.023.
  PUBMED    22980981
REFERENCE   2  (bases 1 to 9577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.08"; journalName: "Cell"; date: "2012-09-14- 
            14"; volume: "150"; issue: "6"; pages: "1209-22"
COMMENT     Sequence Label: FUW-TetO-Zeo-Ezh2
FEATURES             Location/Qualifiers
     source          1..9577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        184..563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        565..763
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             781..961
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1008..1133
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1626..1859
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2044..2088
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2386..2503
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2560..2578
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2602..2620
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2644..2662
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2686..2704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2728..2746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2779..2817
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2814..2838
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(5283..5303)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5288..5617
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5468..5603
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5530..5549
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5665..5712
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5731..6105
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    6189..6777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6242..6262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6660..6671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6780..6796)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6781..6797)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7302..7482
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7508..7525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7514..7625
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(7771..8359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7851..7870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8530..9321,9322..9390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9153..9172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9391..9495)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(9570..9577,1..12))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
       61 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
      121 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      181 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      241 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      301 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      361 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      421 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      481 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      541 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      601 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      661 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      721 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      781 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      841 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      901 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      961 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     1021 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     1081 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     1141 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1201 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1261 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1321 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1381 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1441 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1501 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1561 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1621 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1681 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1741 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1801 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1861 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1921 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1981 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2041 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2101 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2161 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2221 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2281 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2341 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2401 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2461 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2521 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
     2581 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2641 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2701 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2761 gctcggtacc cgggtcgagg taggcgtgta cggtgggagg cctatataag cagagctcgt
     2821 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     2881 caccgggacc gatccagcct ccgcggcccc gaattggaag aataatcatg ggccagactg
     2941 ggaagaaatc tgagaaggga ccggtttgtt ggcggaagcg tgtaaaatca gagtacatga
     3001 gactgagaca gctcaagagg ttcagaagag ctgatgaagt aaagactatg tttagttcca
     3061 atcgtcagaa aattttggaa agaactgaaa ccttaaacca agagtggaag cagcggagga
     3121 tacagcctgt gcacatcatg acttcttgtt cagtcaccag tgacttggat tttccagcac
     3181 aagtcatccc gttaaagacc ctgaatgcag tcgcctcggt gcctataatg tactcttggt
     3241 cgcccttaca acagaatttt atggtggaag acgaaactgt tttacataac attccttata
     3301 tgggggatga agttctggat caggatggca ctttcattga agaactaata aaaaattatg
     3361 atggaaaagt gcatggtgac agagaatgtg gatttataaa tgatgaaatt tttgtggagt
     3421 tggtaaatgc tcttggtcaa tataatgatg atgatgatga cgatgatgga gatgatccag
     3481 atgaaagaga agaaaaacag aaagatctag aggataatcg agatgataaa gaaacttgcc
     3541 cacctcggaa atttcctgct gataaaatat ttgaagccat ttcctcaatg tttccagata
     3601 agggcaccgc agaagaactg aaagaaaaat ataaagaact cacggagcag cagctcccag
     3661 gtgctctgcc tcctgaatgt actccaaaca tcgatggacc aaatgccaaa tctgttcaga
     3721 gggagcaaag cttgcattca tttcatacgc tcttctgtcg acgatgtttt aagtatgact
     3781 gcttcctaca tcccttccat gcaacaccca acacatataa gaggaagaac acagaaacag
     3841 ctttggacaa caagccttgt ggaccacagt gttaccagca tctggaggga gctaaggagt
     3901 ttgctgctgc tcttactgct gagcgtataa agacaccacc taaacgccca gggggccgca
     3961 gaagaggaag acttccgaat aacagtagca gacccagcac ccccaccatc agtgtgctgg
     4021 agtcaaagga tacagacagt gacagagaag cagggactga aactggggga gagaacaatg
     4081 ataaagaaga agaagagaaa aaagatgaga cgtccagctc ctctgaagca aattctcggt
     4141 gtcaaacacc aataaagatg aagccaaata ttgaacctcc tgagaatgtg gagtggagtg
     4201 gtgctgaagc ctccatgttt agagtcctca ttggtactta ctacgataac ttttgtgcca
     4261 ttgctaggct aattgggacc aaaacatgta gacaggtgta tgagtttaga gtcaaggagt
     4321 ccagtatcat agcacctgtt cccactgagg atgtagacac tcctccaaga aagaagaaaa
     4381 ggaaacatcg gttgtgggct gcacactgca gaaagataca actgaaaaag gacggctcct
     4441 ctaaccatgt ttacaactat caaccctgtg accatccacg gcagccttgt gacagttcgt
     4501 gcccttgtgt gatagcacaa aatttttgtg aaaagttttg tcaatgtagt tcagagtgtc
     4561 aaaaccgctt tcctggatgt cggtgcaaag cacaatgcaa caccaaacag tgtccatgct
     4621 acctggctgt ccgagagtgt gaccctgacc tctgtctcac gtgtggagct gctgaccatt
     4681 gggacagtaa aaatgtatcc tgtaagaact gtagcattca gcggggctct aaaaagcact
     4741 tactgctggc accgtctgat gtggcaggct ggggcatctt tatcaaagat cctgtacaga
     4801 aaaatgaatt catctcagaa tactgtgggg agattatttc tcaggatgaa gcagacagaa
     4861 gaggaaaagt gtatgacaaa tacatgtgca gctttctgtt caacttgaac aatgattttg
     4921 tggtggatgc aacccgaaag ggcaacaaaa ttcgttttgc taatcattca gtaaatccaa
     4981 actgctatgc aaaagttatg atggttaatg gtgaccacag gataggcatc tttgctaaga
     5041 gggctatcca gactggtgaa gagttgtttt ttgattacag atacagccag gctgatgccc
     5101 tgaagtatgt gggcatcgaa cgagaaatgg aaatcccttg acatctacta cctcttcccc
     5161 ttctctctga aacagctgcc ttagcttcag gaaccttgag tactgtgggc acaattccca
     5221 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     5281 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagcat
     5341 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5401 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5461 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5521 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     5581 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     5641 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     5701 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     5761 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     5821 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     5881 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     5941 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     6001 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     6061 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     6121 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcaattc gatatcaagc
     6181 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6241 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6301 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6361 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6421 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6481 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6541 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     6601 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     6661 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     6721 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     6781 gataccgtcg acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     6841 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6901 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6961 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7021 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     7081 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7141 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     7201 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     7261 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     7321 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7381 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7441 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     7501 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     7561 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     7621 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     7681 aagggggagg attgggaaga caatagcagg catgtgagca aaaggccagc aaaaggccag
     7741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7981 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8401 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8461 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8521 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8581 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8641 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8701 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8761 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8821 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8881 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8941 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9001 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9061 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9121 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9181 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9241 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9301 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9361 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9421 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9481 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9541 tctcccgatc ccctatggtg cactctcagt acaatct
//
