LOCUS       sequence-129528-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-NGFP-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(36..356)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 ggccctctag atgcatgctc gagttaataa ctagtttcca gttttagaag ctccacatcg
       61 aagacgagag tggcatgtgg tgggatgatg cctgggtgcc cagtggcacc ataggcataa
      121 tctggagata tagtcagttt ggctctctga cccacactca tctgggcaac cccttcttcc
      181 cagcctcgga tcacctcctg cttgcctagc ataaacttaa agggcttgtt tctgtcccgg
      241 gaggaatcaa atttctttcc atcttcaagc atcccggtgt agtgcaccac gcaggtctgg
      301 ccgcgcttgg ggaaggtgcg cccgtctcct ggggagatgg tttccacctg cactcctcta
      361 gtgccgttcc cgttgccgtt gtttctagaa taaagaattg gagcgtaagt ctggtaggta
      421 gctttacgaa catgggaacc caacagagaa agatctctgg cctcaatagt ccactcaaaa
      481 taagctttat ttgaagcctt tctataggat tctactaatt cgttggctct ctcaggcccc
      541 tcagatattg ggtagctctt tgaaacttcc ttgacaagct caacaattct accgtcgggg
      601 gctttctttt ggcccatctc aaaagtaata acttcaaaat attcgacacc cttaatggta
      661 cgagacaaac ggcggacact acgaggtgtt ctaacaacca actcatgggt cgcattacac
      721 gtaaacttga gtaattctgg cacttcacga cttgagtcac ttttgtgggc tctgtgctga
      781 cttttctgca cgacgctgta catagtttct cttcctctgg gcaatttaat tacctcacga
      841 ggtctgccat ctttacc
//
