LOCUS       sequence-129529-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminal portion of GFP along with VMA intein for 
            protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: pcDNA3-FRB-VMAc-CGFP
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..70)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(113..134)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(480..491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctatagaata gggccctcta gatgcatgct cgagttaatt cagatcctct tctgagatga
       61 gtttttgttc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      121 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      181 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      241 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      301 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      361 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gcaattgtgc acgacaacct
      421 ggttggcaag caaaaactga tgatcagaat catcagataa agtaatccca taataatcgt
      481 cttccttcaa ttcttgtaac tcgaaataaa atccgcggca ctcacgtgca aaagcagcgg
      541 cgggagcagg cctgaatttt ttagagccgg cacacttcga aagaacgtta agcaaaacac
      601 tagtctttga gattcgtcgg aacacatgat aatagaggtc ccaggcttgg aggaggtcct
      661 tgacattccc tgatttcatg tacttcctgc accactcttg ggcctccatt aaatctcgac
      721 cataggcctg attaaaggat gtttccttca gagtctgggg gccccgttcc atcatagcat
      781 gcaagggctc cagcacctca aacatgcctt tcacgttcct t
//
