LOCUS       sequence-129530-        5909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
COMMENT     Sequence Label: pAAV-hSyn-RFP
FEATURES             Location/Qualifiers
     source          1..5909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        223..670
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          708..1183
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     1265..1281
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1265..1281
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      1314..1323
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1320..1997
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(1450..1468)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1500..1520)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1718..1737
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1912..1935
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(2039..2057)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2040..2057)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2077..2094)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2083..2194
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2338..2467
                     /label=AAV2 ITR
     rep_origin      2838..3351
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2925..2944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3135..3156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3833..3852)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3952..3974
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4012..4030)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4098..4202
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4203..4271,4272..5063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4421..4440)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5234..5822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5723..5742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
       61 gagcgcagcg agtcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 agaggatcct tcgaaaagct tctgcagagg gccctgcgta tgagtgcaag tgggttttag
      241 gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc cactggacaa
      301 gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga ggggaaacag
      361 gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc cttcgccccc
      421 gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt cactcgccgg
      481 tcccccgcaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg ccggcccagc
      541 cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc tgcgctgcgg
      601 cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg tgtcgtgcct
      661 gagagcgcag tcgaggcgcg ccgagctcgg atcctgagaa cttcagggtg agtctatggg
      721 acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat aggaagggga
      781 gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt aattttaaaa
      841 aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc
      901 tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa
      961 taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc
     1021 atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta
     1081 ccattctgct tttattttat ggttgggata aggctggatt attctgagtc caagctaggc
     1141 ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct
     1201 ggtctgtgtg ctggcccatc actttggcaa agcacgtgaa ttcgaagatc tggccgcctc
     1261 ggcctctaga actagtggat cccccgggct gcaggaattc gatgctaccg gtcgccacca
     1321 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     1381 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1441 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     1501 tgtcccccca gttccagtac ggctccaagg tgtacgtgaa gcaccccgcc gacatccccg
     1561 actacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1621 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggctcc ttcatctaca
     1681 aggtgaagtt catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagacta
     1741 tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga
     1801 tccacaaggc cctgaagctg aaggacggcg gccactacct ggtggagttc aagtccatct
     1861 acatggccaa gaagcccgtg cagctgcccg gctactacta cgtggactcc aagctggaca
     1921 tcacctccca caacgaggac tacaccatcg tggagcagta cgagcgcgcc gagggccgcc
     1981 accacctgtt cctgtagcgg ccaaatgaat tcccaaaccg tacgggaaac ggccctattc
     2041 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     2101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     2161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     2221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcgcgg
     2281 ccgctctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
     2341 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
     2401 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
     2461 cgcgcagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     2521 gcctgaatgg cgaatggcga ttccgttgca atggctggcg gtaatattgt tctggatatt
     2581 accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat tactaatcaa
     2641 agaagtattg cgacaacggt taatttgcgt gatggacaga ctcttttact cggtggcctc
     2701 actgattata aaaacacttc tcaggattct ggcgtaccgt tcctgtctaa aatcccttta
     2761 atcggcctcc tgtttagctc ccgctctgat tctaacgagg aaagcacgtt atacgtgctc
     2821 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2881 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     2941 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3001 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3061 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3121 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3181 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3241 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaaatatt
     3301 tgcttataca atcttcctgt ttttggggct tttctgatta tcaaccgggg tacatatgat
     3361 tgacatgcta gttttacgat taccgttcat cgattctctt gtttgctcca gactctcagg
     3421 caatgacctg atagcctttg tagagacctc tcaaaaatag ctaccctctc cggcatgaat
     3481 ttatcagcta gaacggttga atatcatatt gatggtgatt tgactgtctc cggcctttct
     3541 cacccgtttg aatctttacc tacacattac tcaggcattg catttaaaat atatgagggt
     3601 tctaaaaatt tttatccttg cgttgaaata aaggcttctc ccgcaaaagt attacagggt
     3661 cataatgttt ttggtacaac cgatttagct ttatgctctg aggctttatt gcttaatttt
     3721 gctaattctt tgccttgcct gtatgattta ttggatgttg gaatcgcctg atgcggtatt
     3781 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     3841 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3901 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3961 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     4021 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     4081 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4141 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4201 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4261 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4321 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4381 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4441 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4501 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4561 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4621 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4681 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4741 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4801 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4861 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4921 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4981 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5041 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5101 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5161 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5221 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5281 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5341 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5401 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5461 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5521 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5581 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5641 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5701 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5761 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5821 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5881 tgttctttcc tgcgttatcc cctgattct
//
