LOCUS       sequence-129531-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G 
            mutation along with VMA intein for protein splicing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Alford SC, O'Sullivan C, Obst J, Christie J, Howard PL
  TITLE     Conditional protein splicing of alpha-sarcin in live cells.
  JOURNAL   Mol Biosyst. 2014 Apr;10(4):831-7. doi: 10.1039/c3mb70387h. Epub 
            2014 Jan 30.
  PUBMED    24481070
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c3mb70387h"; journalName: "Mol Biosyst"; date: "2014-04"; 
            volume: "10"; issue: "4"; pages: "831-7"
COMMENT     Sequence Label: 3XFLAG-NSarQ27G-VMAn-FKBP
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(46..366)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 tatagaatag ggccctctag atgcatgctc gagttaataa ctagtttcca gttttagaag
       61 ctccacatcg aagacgagag tggcatgtgg tgggatgatg cctgggtgcc cagtggcacc
      121 ataggcataa tctggagata tagtcagttt ggctctctga cccacactca tctgggcaac
      181 cccttcttcc cagcctcgga tcacctcctg cttgcctagc ataaacttaa agggcttgtt
      241 tctgtcccgg gaggaatcaa atttctttcc atcttcaagc atcccggtgt agtgcaccac
      301 gcaggtctgg ccgcgcttgg ggaaggtgcg cccgtctcct ggggagatgg tttccacctg
      361 cactcctcta gtgccgttcc cgttgccgtt gtttctagaa taaagaattg gagcgtaagt
      421 ctggtaggta gctttacgaa catgggaacc caacagagaa agatctctgg cctcaatagt
      481 ccactcaaaa taagctttat ttgaagcctt tctataggat tctactaatt cgttggctct
      541 ctcaggcccc tcagatattg ggtagctctt tgaaacttcc ttgacaagct caacaattct
      601 accgtcgggg gctttctttt ggcccatctc aaaagtaata acttcaaaat attcgacacc
      661 cttaatggta cgagacaaac ggcggacact acgaggtgtt ctaacaacca actcatgggt
      721 cgcattacac gtaaacttga gtaattctgg cacttcacga cttgagtcac ttttgtgggc
      781 tctgtgctga cttttctgca cgacgctgta catagtttct cttcctctgg gcaatt
//
