LOCUS       sequence-129533-        6777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Nathanson JL, Yanagawa Y, Obata K, Callaway EM
  TITLE     Preferential labeling of inhibitory and excitatory cortical neurons 
            by endogenous tropism of adeno-associated virus and lentivirus 
            vectors.
  JOURNAL   Neuroscience. 2009 Jun 30. 161(2):441-50.
  PUBMED    19318117
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroscience. 2009 Jun 30. 161(2):441-50."
COMMENT     Sequence Label: pAAV-mCAMK-RFP
FEATURES             Location/Qualifiers
     source          1..6777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        214..1502
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     intron          1582..2057
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     2139..2155
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2871
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(2324..2342)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(2374..2394)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2592..2611
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2786..2809
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        complement(2913..2931)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2914..2931)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2951..2968)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2957..3068
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3212..3341
                     /label=AAV2 ITR
     rep_origin      3712..4225
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3799..3818)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4009..4030
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4707..4726)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4826..4848
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4886..4904)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4972..5076
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5077..5145,5146..5937)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5295..5314)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6108..6696
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6597..6616
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
       61 acgaccgagc gcagcgagtc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagag gatccttcga aaagcttgta taacattatg gccttaggtc acttcatctc
      241 catggggttc ttcttctgat tttctagaaa atgagatggg ggtgcagaga gcttcctcag
      301 tgacctgccc agggtcacat cagaaatgtc agagctagaa cttgaactca gattactaat
      361 cttaaattcc atgccttggg ggcatgcaag tacgatatac agaaggagtg aactcattag
      421 ggcagatgac caatgagttt aggaaagaag agtccagggc agggtacatc tacaccaccc
      481 gcccagccct gggtgagtcc agccacgttc acctcattat agttgcctct ctccagtcct
      541 accttgacgg gaagcacaag cagaaactgg gacaggagcc ccaggagacc aaatcttcat
      601 ggtccctctg ggaggatggg tggggagagc tgtggcagag gcctcaggag gggccctgct
      661 gctcagtggt gacagatagg ggtgagaaag cagacagagt cattccgtca gcattctggg
      721 tctgtttggt acttcttctc acgctaaggt ggcggtgtga tatgcacaat ggctaaaaag
      781 cagggagagc tggaaagaaa caaggacaga gacagaggcc aagtcaacca gaccaattcc
      841 cagaggaagc aaagaaacca ttacagagac tacaaggggg aagggaagga gagatgaatt
      901 agcttcccct gtaaacctta gaacccagct gttgccaggg caacggggca atacctgtct
      961 cttcagagga gatgaagttg ccagggtaac tacatcctgt ctttctcaag gaccatccca
     1021 gaatgtggca cccactagcc gttaccatag caactgcctc tttgccccac ttaatcccat
     1081 cccgtctgtt aaaagggccc tatagttgga ggtgggggag gtaggaagag cgatgatcac
     1141 ttgtggacta agtttgttcg catccccttc tccaaccccc tcagtacatc accctggggg
     1201 aacagggtcc acttgctcct gggcccacac agtcctgcag tattgtgtat ataaggccag
     1261 ggcaaagagg agcaggtttt aaagtgaaag gcaggcaggt gttggggagg cagttaccgg
     1321 ggcaacggga acagggcgtt tcggaggtgg ttgccatggg gacctggatg ctgacgaagg
     1381 ctcgcgaggc tgtgagcagc cacagtgccc tgctcagaag ccccaagctc gtcagtcaag
     1441 ccggttctcc gtttgcactc aggagcacgg gcaggcgagt ggcccctagt tctgggggca
     1501 gcgggggatc ccctacggcc ggccacgcgt tcgcgaccgc gggctagcgg cgcgccgagc
     1561 tcggatcctg agaacttcag ggtgagtcta tgggaccctt gatgttttct ttccccttct
     1621 tttctatggt taagttcatg tcataggaag gggagaagta acagggtaca catattgacc
     1681 aaatcagggt aattttgcat ttgtaatttt aaaaaatgct ttcttctttt aatatacttt
     1741 tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca ataatgatac
     1801 aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc tgggttaagg
     1861 caatagcaat atttctgcat ataaatattt ctgcatataa attgtaactg atgtaagagg
     1921 tttcatattg ctaatagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg
     1981 gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc
     2041 ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg
     2101 gcaaagcacg tgaattcgaa gatctggccg cctcggcctc tagaactagt ggatcccccg
     2161 ggctgcagga attcgatgct accggtcgcc accatggcct cctccgagga cgtcatcaag
     2221 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
     2281 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2341 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     2401 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     2461 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     2521 gactcctccc tgcaggacgg ctccttcatc tacaaggtga agttcatcgg cgtgaacttc
     2581 ccctccgacg gccccgtaat gcagaagaag actatgggct gggaggcctc caccgagcgc
     2641 ctgtaccccc gcgacggcgt gctgaagggc gagatccaca aggccctgaa gctgaaggac
     2701 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     2761 cccggctact actacgtgga ctccaagctg gacatcacct cccacaacga ggactacacc
     2821 atcgtggagc agtacgagcg cgccgagggc cgccaccacc tgttcctgta gcggccaaat
     2881 gaattcccaa accgtacggg aaacggccct attctatagt gtcacctaaa tgctagagct
     2941 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3001 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3061 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3121 agcaaggggg aggattggga agacaatagc gcggccgctc tagagcatgg ctacgtagat
     3181 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     3241 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     3301 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctggcgtaa tagcgaagag
     3361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgattccgt
     3421 tgcaatggct ggcggtaata ttgttctgga tattaccagc aaggccgata gtttgagttc
     3481 ttctactcag gcaagtgatg ttattactaa tcaaagaagt attgcgacaa cggttaattt
     3541 gcgtgatgga cagactcttt tactcggtgg cctcactgat tataaaaaca cttctcagga
     3601 ttctggcgta ccgttcctgt ctaaaatccc tttaatcggc ctcctgttta gctcccgctc
     3661 tgattctaac gaggaaagca cgttatacgt gctcgtcaaa gcaaccatag tacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4081 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 ttaacaaaat attaacgctt acaatttaaa tatttgctta tacaatcttc ctgtttttgg
     4201 ggcttttctg attatcaacc ggggtacata tgattgacat gctagtttta cgattaccgt
     4261 tcatcgattc tcttgtttgc tccagactct caggcaatga cctgatagcc tttgtagaga
     4321 cctctcaaaa atagctaccc tctccggcat gaatttatca gctagaacgg ttgaatatca
     4381 tattgatggt gatttgactg tctccggcct ttctcacccg tttgaatctt tacctacaca
     4441 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga
     4501 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt
     4561 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga
     4621 tttattggat gttggaatcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4681 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4741 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4801 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4861 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     4921 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     4981 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5041 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5101 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5161 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5221 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5281 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5341 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5401 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5461 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5521 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5581 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5641 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5701 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5761 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5821 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     5881 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     5941 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6001 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6061 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6721 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccct
//
