LOCUS       sequence-129538-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Liu F, Wagner S, Campbell RB, Nickerson JA, Schiffer CA, Ross AH
  TITLE     PTEN enters the nucleus by diffusion.
  JOURNAL   J Cell Biochem. 2005 Oct 1. 96(2):221-34.
  PUBMED    16088943
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biochem. 2005 Oct 1. 96(2):221-34."
COMMENT     Sequence Label: GFP-PTEN
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(101..613,614..616,617..619))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     294..313
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     553..574
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(661..680)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(662..680)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
       61 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
      121 tgaagttcac cttgatgccg ttcttctgct tgtcggccgt gatatagacg ttgtggctgt
      181 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
      241 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
      301 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
      361 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac tgcacgccgt
      421 agccgaaggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
      481 acttcagggt cagcttgccg tangtggcat cgccctcgcc ctcgccggac acgctgaact
      541 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
      601 cctcgccctt gctcaccatg gcagtctaga gtcgacctgc aggcatgcaa gcttgggtct
      661 ccctatagtg agtcgtatta atttcgataa gccagtaagc
//
