LOCUS       sequence-129544-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses p53 tagged with mKate2 and split N-term mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..52
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttctccttaa tcagctcaga caccattccc actttgtaca agaaagctgg gtcgtctgag
       61 tcaggccctt ctgtcttgaa catgagtttt ttatggcggg aggtagactg accctttttg
      121 gacttcaggt ggctggagtg agccctgctc ccccctggct ccttcccagc ctgggcatcc
      181 ttgagttcca aggcctcatt cagctctcgg aacatctcga agcgctcacg cccacggatc
      241 tgaagggtga aatattctcc atccagtggt ttcttctttg gctggggaga ggagctggtg
      301 ttgttgggca gtgctcgctt agtgctccct gggggcagct cgtggtgagg ctcccctttc
      361 ttgcggagat tctcttcctc tgtgcgccgg tctctcccag gacaggcaca aacacgcacc
      421 tcaaagctgt tccgtcccag taggtttcca gagctatcct ccagtgtgat gatggtgagg
      481 atgggcctcc ggttcatgcc gcccatgcag gaactgttac acatgtagtt gtagtggatg
      541 gtggtacagt cag
//
