LOCUS       sequence-129553-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo. Cre inducible expression 
            (double floxed inversed ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon 
            Wiegert J
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo.
  JOURNAL   Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
  PUBMED    26443033
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-7"; volume: "5:14807. doi"; pages: " 
            10.1038/srep14807"
COMMENT     Sequence Label: pAAV EF1a DIO iChloC 2A dsRed
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(34..67)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(118..151)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(160..840)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MVASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFL"
     primer_bind     complement(222..245)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(420..439)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     637..657
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     689..707
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      833..842
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaggtacg gatcctctag agtcgactcc ggaataactt cgtataggat actttatacg
       61 aagttatgca gaatggtagc tggattgtag ctgctattag caatatgaaa cctcttaata
      121 acttcgtata gcatacatta tacgaagtta tggcgcgccc tacaggaaca ggtggtggcg
      181 gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      241 cagcttggtg tccacgtagt agtagccggg cagctgcacg ggcttcttgg ccatgtagat
      301 ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc
      361 gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt
      421 cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcggaact tcaccttgta
      481 gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
      541 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtagtcggg
      601 gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac tggaactggg gggacaggat
      661 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
      721 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
      781 ctccatgcgc accttgaagc gcatgaactc tttgatgacg tcctcggagg aggccaccat
      841 ggcggccgcg gatcccggtg cagggccggg attctcctcc acgtcaccg
//
