LOCUS       sequence-129554-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo. Cre inducible expression 
            (double floxed inversed ORF).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner TG, Simon 
            Wiegert J
  TITLE     An improved chloride-conducting channelrhodopsin for light-induced 
            inhibition of neuronal activity in vivo.
  JOURNAL   Sci Rep. 2015 Oct 7;5:14807. doi: 10.1038/srep14807.
  PUBMED    26443033
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-10-7"; volume: "5:14807. doi"; pages: " 
            10.1038/srep14807"
COMMENT     Sequence Label: pAAV EF1a DIO iChloC 2A dsRed
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(132..165)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      170..179
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aattttgtat ccagaggttg attatcgata agcttgatat cgaattcata acttcgtata
       61 gcatacatta tacgaagtta ttctttgcac cattctaaag aataacagtg ataatttctg
      121 ggttaaggca aataacttcg tataggatac tttatacgaa gttatgctag ccaccatgga
      181 ttatggaggc gccctgagtg ccgttgggcg cgagctgcta tttgtaacga acccagtagt
      241 cgtcaatggc tctgtacttg tgcctgagga ccagtgttac tgcgcgggct ggattgagtc
      301 gcgtggcaca aacggtgccc aaacggcgtc gaacgtgctg caatggcttg ctgctggctt
      361 ctccatccta ctgcttatgt tttacgccta ccaaacatgg aagtcaacct gcggctggga
      421 gcagatctat gtgtgcgcta tcaggatggt caaggtgatt ctcgagttct tcttctcgtt
      481 taagaacccg tccatgctgt atctagccac aggccaccgc gtccagtggt tgcgttacgc
      541 cgagtggctt ctcacctgcc cggtcattct cattcacctg tcaaacctga cgggcttgtc
      601 caacgactac agcaggcgca ccatgggtct gcttgtgtct aatattggct gtattgtgtg
      661 gggcgccact tccgccatgg ccaccggata cgtcaaggtc atcttcttct gcctgggtct
      721 gtgttatggt gctaacacgt tctttcacgc tgccaaggcc tacatcgagg gttaccacac
      781 cgtgccgaag ggccggtgtc gccaggtggt gactggcatg gcttggctct tcttcgtatc
      841 atggg
//
