LOCUS       sequence-129562-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chloride-conducting Channelrhodopsin with slow kinetics, 
            neuron-specific promoter, plus red fluorescent protein, excitatory 
            neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(50..724)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(112..135)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(310..329)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     527..547
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     579..597
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttgtaatc cagaggttga ttatcgataa gcttttgctc gaagtcgacc tacaggaaca
       61 ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg
      121 aggtgatgtc cagcttggtg tccacgtagt agtagccggg cagctgcacg ggcttcttgg
      181 ccatgtagat ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct
      241 ggtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc
      301 agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcggaact
      361 tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc
      421 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct
      481 tgtagtcggg gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac tggaactggg
      541 gggacaggat gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg
      601 tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt
      661 tcacggagcc ctccatgcgc accttgaagc gcatgaactc tttgatgacg tcctcggagg
      721 aggcggtgcc ggagctgccg ctgccggtgc tgccggtgcc atgcccc
//
