LOCUS       sequence-129570-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expession of GFP-CtlP T847E mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker 
            A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, 
            Durocher D
  TITLE     A mechanism for the suppression of homologous recombination in G1 
            cells.
  JOURNAL   Nature. 2015 Dec 9. doi: 10.1038/nature16142.
  PUBMED    26649820
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Dec 9. doi: 10.1038/nature16142."
COMMENT     Sequence Label: pCW-GFP-CtIP-T847E
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..97)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    115..139
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa
       61 ccccaacccc ggatccttag tggtggtggt ggtggtggac cggttcatta ctaaaccact
      121 ttgtacaaga aagctgggtc ctatgtcttc tgctccttgc cttttggaga aaatattgcg
      181 ttgtaaggct gacgtctttt tggacgagga caaggatcaa gatcttcctt aatataacct
      241 ctttccatac aagtctgagt ggaaggaaaa ccaacttccc aaaaattctc tggctcgttg
      301 ggtgggatgt agcggaatcg gtgtcttgag caggaagcca atttcttttc tctttcttct
      361 gctggcatat ctgcataata aatttcacat tccttacacg tgtgcccaag cagttttctt
      421 ctctcctctt ttttccgaac cacctcaata tgaggaaaat tttgcaagct agtctctctt
      481 tcatcacctt taaaatacgg ctccacaaac gctttctgct tgactttgtc ttgtttatca
      541 ccatgagtgt gtagtttctt tgtggcagta gacaattcct cctcttcatc tgctgcttgg
      601 gagaaactgt ctgccaaaca ggattcatac tcttcatgtg ttgtccgatc aaacatatct
      661 tccaagctat cattcatttt tctttcttca tttgaacttt tttctccctt ctgctcttgt
      721 ttcttcattt ttaagagaac ggtttcacta accaatgtac agtccatgtc tactgtctct
      781 cctcctaatt ttgactgact gccatccttt gtatctatta cagtaacatc catt
//
