LOCUS       sequence-129572-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Historical plasmid. For inhibition of neuronal activity, note that 
            Addgene Plasmid #66709 is a light-gated chloride channel with 
            improved anion selectivity (iChloC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV Syn ChR E90R-T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(64..738)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(126..149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(324..343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     541..561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     593..611
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcatctttca caaattttgt aatccagagg ttgattatcg ataagctttt gctcgaagtc
       61 gacctacagg aacaggtggt ggcggccctc ggagcgctcg tactgttcca cgatggtgta
      121 gtcctcgttg tgggaggtga tgtccagctt ggtgtccacg tagtagtagc cgggcagctg
      181 cacgggcttc ttggccatgt agatggtctt gaactccacc aggtagtggc cgccgtcctt
      241 cagcttcagg gcctggtgga tctcgccctt cagcacgccg tcgcgggggt acaggcgctc
      301 ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg gggggaagtt
      361 ggtgccgcgg aacttcacct tgtagatcag cgtgccgtcc tgcagggagg agtcctgggt
      421 cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga agccctcggg
      481 gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtacg ccttggagcc
      541 gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc ccttggtcac
      601 cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc cctcgatctc
      661 gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga actctttgat
      721 gacgtcctcg gaggaggcgg tgccggagct gccgctgccg gtgctgccgg tgccatg
//
