LOCUS       sequence-129574-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Historical plasmid. For inhibition of neuronal activity, note that 
            Addgene Plasmid #66709 is a light-gated chloride channel with 
            improved anion selectivity (iChloC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Wietek J, Wiegert JS, Adeishvili N, Schneider F, Watanabe H, Tsunoda
            SP, Vogt A, Elstner M, Oertner TG, Hegemann P
  TITLE     Conversion of Channelrhodopsin into a Light-Gated Chloride Channel.
  JOURNAL   Science. 2014 Mar 27.
  PUBMED    24674867
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2014 Mar 27."
COMMENT     Sequence Label: pAAV Syn ChR E90R-D156N-T159C 2A tDimer
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..726)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(114..137)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(312..331)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     529..549
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     581..599
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttttgc tcgaagtcga cctacaggaa
       61 caggtggtgg cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg
      121 ggaggtgatg tccagcttgg tgtccacgta gtagtagccg ggcagctgca cgggcttctt
      181 ggccatgtag atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc
      241 ctggtggatc tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc
      301 ccagcccatg gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcggaa
      361 cttcaccttg tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccac
      421 gccgccgtcc tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt
      481 cttgtagtcg gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt actggaactg
      541 gggggacagg atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc
      601 ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc
      661 gttcacggag ccctccatgc gcaccttgaa gcgcatgaac tctttgatga cgtcctcgga
      721 ggaggcggtg cc
//
