LOCUS       sequence-129585-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pRipTAL plasmid compatible with Golden Gate TALEN cloning system. 
            For cloning of megaTALs or other TAL-fusions and expression in 
            mammalian cells. Meganuclease/fusion is cloned between Xba-Sal 
            sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  TITLE     TALEN Golden Gate vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    449..470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(485..490,491..508,509..515)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    523..539
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     528..550
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     547..563
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     547..563
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             559..1008
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TGKLPSQPIQGLDCWPLSVLGHCGDLGGGQPQRGGSKCGSHRQQGL"
     primer_bind     582..602
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        584..602
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(649..668)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(650..668)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(678..695)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(678..694)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(687..709)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt aacaggaaca gagcgtaaat
       61 aacgggaaag tttcttaaca tgtttgtctt gtggcaatac acctgaacta gtaattacat
      121 atccctaaaa atgtaaatga ttgccccacc attttgttct attaacattt aaatgtatac
      181 ccaaatcaag aaaaacagaa caaatatggg tataaatggc ggtaagatgc tcggtcgact
      241 gagatcagat cactacgtca tctagatatc gcaacgcgat gggacgtgcg ttcgccaata
      301 cggcgattga ctcttctgat caattccggc gcgtgcggca atcccttttt cactgcatcc
      361 atggcaggac gtccgccgag gcaggccaag gcgacgaggt ggtcgttgga gacgaccggt
      421 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      481 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      541 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      601 gggaacaaaa gctggaggac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
      661 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      841 ccctgtagcg gcgcccgtct ccaggggggc accggtaagc ttccttcgca gcctatacag
      901 ggcttggatt gctggccgct ctccgtactg ggccactgtg gcgatctggg aggcggacag
      961 ccgcagcggg gcggcagtaa gtgcggtagc cacaggcagc agggcttgga
//
