LOCUS       sequence-129586-         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein production from a synthetic gene encoding Treponema 
            denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Tranchimand S, Starks CM, Mathews II, Hockings SC, Kappock TJ
  TITLE     Treponema denticola PurE Is a bacterial AIR carboxylase.
  JOURNAL   Biochemistry. 2011 May 31;50(21):4623-37. doi: 10.1021/bi102033a. 
            Epub 2011 May 6.
  PUBMED    21548610
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi102033a";
            journalName: "Biochemistry"; date: "2011-05-31- 31"; volume: "50"; 
            issue: "21"; pages: "4623-37"
COMMENT     Sequence Label: pJK376
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(41..496)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(178..199)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     390..409
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(533..580)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     576..594
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(647..664)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
       61 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
      121 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
      181 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
      241 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
      301 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
      361 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
      421 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
      481 ccgctacagg gcgcgtccca ttcgccaatc cggatatagt tcctcctttc agcaaaaaac
      541 ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca gcggtggcag
      601 cagccaactc agcttccttt cgggctttgt tagcagccgg atctcagtgg tggtggtggt
      661 ggtgctcgag tgcggccgca agcttgtcga cgga
//
