LOCUS       sequence-129589-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  TITLE     pZac2.1gfaABC1Dlck-GCaMP6f
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-lck-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        29..47
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      169..178
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             184..201
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             205..237
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(241..249,250..264)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             292..348
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     561..582
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(688..709)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcctcgagaa
       61 ttcaccatgg gctgtggctg cagctcaaac cctgaagatg actggatgga gaacattgac
      121 gtgtgtgaga actgccatta tcccccgccg aagcttcgaa tggatccagc caccatgggt
      181 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg
      241 gatctgtacg acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag
      301 tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc
      361 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
      421 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac
      481 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccaaact ttcgaaagac
      541 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      601 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag
      661 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      721 aagttcagc
//
