LOCUS       sequence-129590-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for calcium signaling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Haustein MD, Kracun S, Lu XH, Shih T, Jackson-Weaver O, Tong X, Xu 
            J, Yang XW, O'Dell TJ, Marvin JS, Ellisman MH, Bushong EA, Looger 
            LL, Khakh BS
  TITLE     Conditions and constraints for astrocyte calcium signaling in the 
            hippocampal mossy fiber pathway.
  JOURNAL   Neuron. 2014 Apr 16;82(2):413-29. doi: 10.1016/j.neuron.2014.02.041.
  PUBMED    24742463
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2014.02"; journalName: "Neuron"; date: 
            "2014-04-16- 16"; volume: "82"; issue: "2"; pages: "413-29"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-cyto-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      71..80
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             86..103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             107..139
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(143..151,152..166)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             200..256
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     469..490
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(596..617)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(857..876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
       61 ataggctagc gccaccatgg gttctcatca tcatcatcat catggtatgg ctagcatgac
      121 tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggatc tcgctagcgc
      181 caccatggtc gactcatcac gtcgtaagtg gaataagaca ggtcacgcag tcagagctat
      241 aggtcggctg agctcactcg agaacgtcta tatcaaggcc gacaagcaga agaacggcat
      301 caaggcgaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
      361 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      421 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
      481 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
      541 cggagggagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      601 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
      661 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      721 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
      781 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
      841 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      901 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      961 cctggggcac aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga
     1021 atttaaagag gaattctccc tatttgacaa ggacggg
//
