LOCUS       sequence-129600-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Rci gene (driven by the inducible T7 promoter) and 5 
            fragment barcode cassette in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Peikon ID, Gizatullina DI, Zador AM
  TITLE     In vivo generation of DNA sequence diversity for cellular barcoding.
  JOURNAL   Nucleic Acids Res. 2014;42(16):e127. doi: 10.1093/nar/gku604. Epub 
            2014 Jul 10.
  PUBMED    25013177
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2014"; volume: "42(16):e127. doi"; pages: " 
            10.1093/nar/gku604. Epub 2014 Jul 10"
COMMENT     Sequence Label: IDP205 (T7->Rci; 5bc)
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata catatgccgt
      481 ctccacgcat ccgtaaaatg tccctgtcac gcgcactgga taagtacctg aaaacagttt
      541 ctgttcacaa gaaagggcat caacaggagt tttaccggag caatgttatc aagcgatatc
      601 ccattgctct tcggaatatg gacgaaataa caaccgttga tattgctaca tacagagacg
      661 ttcgtttagc agaaataaac ccccgaacgg gtaaagccat tacaggtaat actgtacgtc
      721 ttgaactcgc ccttctgtca tctctgttca atattgctcg tgttgaatgg ggaacctgtc
      781 gtactaaccc ggttgaactg gttcgcaagc cgaaagtatc ctccggacga gatcgccggc
      841 taacgtcttc agaagaacgt cgcctttctc gctatttccg cgaaaaaaat ctgatgttgt
      901 atgtcatttt ccatcttgcc cttgaaacag ccatgcggca gggcgaaata ctggccttac
      961 gttgggagca cattgatttg cgccacggtg tggctcattt acctgaaacc aaa
//
