LOCUS       sequence-129602-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Rci gene (driven by the inducible T7 promoter) and 5 
            fragment barcode cassette in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Peikon ID, Gizatullina DI, Zador AM
  TITLE     In vivo generation of DNA sequence diversity for cellular barcoding.
  JOURNAL   Nucleic Acids Res. 2014;42(16):e127. doi: 10.1093/nar/gku604. Epub 
            2014 Jul 10.
  PUBMED    25013177
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2014"; volume: "42(16):e127. doi"; pages: " 
            10.1093/nar/gku604. Epub 2014 Jul 10"
COMMENT     Sequence Label: IDP205 (T7->Rci; 5bc)
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(96..118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    204..343
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     259..278
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(358..375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
       61 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      181 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
      241 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac cattgcggtg
      301 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
      361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgttgaggca
      481 aggaagatgc tgtccggcaa tactttcgtg ccaatccggt acgtggccaa ttggtagttt
      541 gtccagaact cagattttaa cagcagagga cgcatgctct accttcatga tccactgacg
      601 tccctgaggc tgcaatacat gcaacgccac gtaccggatt ggcacgtagg ccagcagagg
      661 cagtctccgc ggtaagtcct agtgcaatgg cgctttttta ccctcgtcct cgagaagagg
      721 ggacgccagt gcagatatct ttaatgtggt aattggggca cgtaggccag tgccaatccg
      781 gtacctggag gactcttggc cctccgccct taggcagtgc atactcttcc ataaacgggc
      841 tgttagttat ggcgtccgag gattcaaaaa ggtgagcgaa ctcggccgcc acgtaccgga
      901 ttggcacaac aagcttggca tccggagaga cgggcttcaa agctgcctga cgacggttgc
      961 gcgtccg
//
