LOCUS       sequence-129605-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cobalamin riboswitch reporter vector for use in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Johnson JE Jr, Reyes FE, Polaski JT, Batey RT
  TITLE     B12 cofactors directly stabilize an mRNA regulatory switch.
  JOURNAL   Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 
            2012 Oct 14.
  PUBMED    23064232
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11607"; journalName: "Nature"; date: "2012-12-6- 6"; 
            volume: "492"; issue: "7427"; pages: "133-7"
COMMENT     Sequence Label: pENV8(WT)-GFPuv
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     578..606
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     terminator      complement(930..957)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gttgggatct ttcgaaaggg cagattgtgt cgacaggtaa tggttgtctg gtaaaaggac
       61 agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa cggatccatc
      121 ttcaatgttg tggcgaattt tgaagttagc tttgattcca ttcttttgtt tgtctgccgt
      181 gatgtataca ttgtgtgagt tatagttgta ctcgagtttg tgtccgagaa tgtttccatc
      241 ttctttaaaa tcaatacctt ttaactcgat acgattaaca agggtatcac cttcaaactt
      301 gacttcagca cgcgtcttgt agttcccgtc atctttgaaa gatatagtgc gttcctgtac
      361 ataaccttcg ggcatggcac tcttgaaaaa gtcatgccgt ttcatatgat ccggataacg
      421 ggaaaagcat tgaacaccat aagagaaagt agtgacaagt gttggccatg gaacaggtag
      481 ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc
      541 ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg
      601 gacaactcca gtgaaaagtt cttctccttt actcattttt tctaccggta cccggggatc
      661 ctctagagtc gacctgcagg catgcaagct tggcgtaatc atcagcatgt tgagtctcct
      721 tgctcgttgt atggcctagg tggcattcgg agtataaccg tatcaagtaa tctggacgaa
      781 tttcacgtca ctttcccact acgcttttag gccattatta tatgaatata ttatacgagc
      841 cttatgcatg cccgtaaagt tatccagcaa ccactcatag acctagggca gcagataggg
      901 acgacgtggt gttagctgtg gctagcgcaa aaaggccatc cgtcaggatg gccttctgct
      961 taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     1021 tc
//
