LOCUS       sequence-129607-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control cobalamin riboswitch reporter vector for use in E. 
            coli; deletion of regulatory kissing loop interaction by conversion 
            of L5 to GAAA tetra loop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Johnson JE Jr, Reyes FE, Polaski JT, Batey RT
  TITLE     B12 cofactors directly stabilize an mRNA regulatory switch.
  JOURNAL   Nature. 2012 Dec 6;492(7427):133-7. doi: 10.1038/nature11607. Epub 
            2012 Oct 14.
  PUBMED    23064232
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature11607"; journalName: "Nature"; date: "2012-12-6- 6"; 
            volume: "492"; issue: "7427"; pages: "133-7"
COMMENT     Sequence Label: pENV8(GAAA)-GFPuv
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(473..492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     592..614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(779..799)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggccctta aggccgcctg atgcggtatt
      421 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
      481 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      541 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      601 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcccgtacg
      661 gccgactagt aggcctatta tttttgacac cagacaagtt ggtaatggta gcgaccggcg
      721 ctcagttgga attcattatt tgtagagctc atccatgcca tgtgtaatcc cagcagcagt
      781 tacaaactca agaaggacca tgtggtcacg cttttcgttg ggatctttcg aaagggcaga
      841 ttgtgtcgac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt
      901 ttgttgataa tggtctgcta gttgaacgga tccatcttca atgttgtggc gaattttgaa
      961 gttagctttg attccattct tttgtttgtc tgccgtgatg tatacattgt gtgagttata
     1021 gttgtactcg agtttgtgtc cgagaatgtt tccatcttct ttaaaatcaa taccttttaa
     1081 ctcgatacga ttaacaaggg
//
