LOCUS       sequence-129608-         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Rci gene (driven by the inducible T7 promoter) and 11 
            fragment barcode cassette in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Peikon ID, Gizatullina DI, Zador AM
  TITLE     In vivo generation of DNA sequence diversity for cellular barcoding.
  JOURNAL   Nucleic Acids Res. 2014;42(16):e127. doi: 10.1093/nar/gku604. Epub 
            2014 Jul 10.
  PUBMED    25013177
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2014"; volume: "42(16):e127. doi"; pages: " 
            10.1093/nar/gku604. Epub 2014 Jul 10"
COMMENT     Sequence Label: DIG70 (T7->Rci; 11bc)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgagtgcg gccgcttaca gcggtgtgct gcccgggttc agagggcaaa tcataatgta
      181 gtcagggtcg ttaacaggta acgctccggg agatgggaca cgttcgcctt tctgtgctgc
      241 aatggccagt gtacgcaaca aaacctcact ggcgcggtga actgcttcct cttttgttgt
      301 ggcagtgacg tgaaggttat caaaatcacc gatctcaata cgatgcgctt tctgcccatt
      361 ttcttcatcg atagtcgtga tatgggcagg atacggcaca aaccatgctg ccactttttg
      421 tgtctgccgc cggcgggcat caagcttact gaccagttgc catgcacgaa gatgagtata
      481 ccgtttcagc atattcatgg aacgatgtcc tgatattgca gcaatctcca ttacattcag
      541 gctacccagt tcgaagaagc ggcttattgc ttcatgccgt agatcgtgaa aatgcaggtc
      601 ctcgatgcga agtcgttgtg tggctattct ccaggcattt ttaaagccgg atgcggtgta
      661 atcaaaaaca ttgccgtgga gattaacggg catcatttga agaaagttac gggcacgtct
      721 ggacagagga acatcccgtg agtgaccgtt tttggtttca ggtaaatgag ccacaccgtg
      781 gcgcaaatca atgtgctccc aacgtaaggc cagtatttcg ccctgccgca tggctgtttc
      841 aagggcaaga tggaaaatga catacaacat cagattttt
//
