LOCUS       sequence-129612-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used as a template for in vitro transcription of Cas9 mRNA after 
            linearizing with BbsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Karl Degenhardt
  TITLE     Degenhardt lab Cas9 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: TC9pAB
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      67..76
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             79..99
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tagtaacggc cgccagtgtg ctggaattcg cccttaggaa ttaatacgac tcactatagg
       61 gtgaccgcca ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
      121 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      181 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      241 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      301 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      361 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
      421 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
      481 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
      541 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
      601 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
      661 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
      721 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
      781 cggctggaa
//
