LOCUS       sequence-129614-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ustilago maydis codon-optimized Cas9, contains U. maydis 
            U6 promoter, is self-replicating.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Schuster M, Schweizer G, Reissmann S, Kahmann R
  TITLE     Genome editing in Ustilago maydis using the CRISPR-Cas system.
  JOURNAL   Fungal Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 
            10.1016/j.fgb.2015.09.001.
  PUBMED    26365384
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 
            10.1016/j.fgb.2015.09.001."
COMMENT     Sequence Label: pCas9_sgRNA_0
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(264..311)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(321..347)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat
       61 cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt
      121 catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat
      181 catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg
      241 atctgcagcc gggcggccgc tcacttcttc ttcttggcct gtccagcctt cttagtggct
      301 gcgggacgct ttccgccgct agcgtaatcg ggcacatcgt atgggtagcc gccgctgtca
      361 ccgcccagtt gagacagatc gatgcgggtt tcgtacagac cggtgatcga ctgatggatc
      421 agtgtagcgt cgaggacttc cttcgtgctt gtgtaacgct tgcggtcgat ggtcgtgtca
      481 aagtatttaa acgccgcagg cgcacccaag ttagtgagag tgaagaggtg gatgatgttc
      541 tcggcctgct cgcgaatcgg cttatctcga tgcttgttgt aggcagacaa caccttgtcg
      601 aggttggcat cagccaagat cactcgcttg ctaaactccg agatttgctc gatgatttca
      661 tccaggtaat gtttgtgttg ctccacgaag agctgctttt gttcgttgtc ctcggggcta
      721 cccttcaact tctcataatg gcttgcgagg tagaggaagt tgacgtactt ggagggcaa
//
