LOCUS       sequence-129616-         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses Ustilago maydis codon-optimized Cas9, contains U. maydis 
            U6 promoter, is self-replicating.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Schuster M, Schweizer G, Reissmann S, Kahmann R
  TITLE     Genome editing in Ustilago maydis using the CRISPR-Cas system.
  JOURNAL   Fungal Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 
            10.1016/j.fgb.2015.09.001.
  PUBMED    26365384
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 Sep 11. pii: S1087-1845(15)30025-6. doi: 
            10.1016/j.fgb.2015.09.001."
COMMENT     Sequence Label: pCas9_sgRNA_0
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 acagctatga ccatgattac gccaagcttt aatacgttcg ttccgatgta gctcaggaac
       61 gggcgcagag gcggatgaat tggttgcggt ggctatggat ggtggaggtg aacggcgacg
      121 tgcagcgtcg gtcacagagg atcggatggg gcctgtcggg atgacctgta ctataacgag
      181 ggaggagggg gagggaggag gggaaagagg aatgtgggga aggcacgtta ccaaggtttt
      241 gccaagaggc tcttgtgtta ttccggaggt gtagccgaat gggcgatccg agctaacgcc
      301 agctgggcgt gagaagcagt tggtctgaaa gcggaggcgg tgcaagacgg ttctaagaag
      361 gaggcgaata agaagtgttt tgtgtgctgc gggtgcgaat agacggtcac gagtggatgg
      421 aagccgactt gtaggcgtgc tgaaagacgt cgtgcgggtg cggttttggt tttgtgttgg
      481 tcttggtaaa agtgtgccgc aggtgagggt tcttgattgg tgaacgtgaa aacggatggc
      541 caagtccgag tcgaccagag agagaggcag agagagagag agagatggct cttcagcgcc
      601 gcttcgcgcg tccttcacga ttattcgtga ttactgtcca ccggtccctc ttactcagaa
      661 ctgccggaac gaattcgtga tttacaccaa acacgcgctg tcacagtcac gagtccatga
      721 gccgtgagcc ccgcttcaga tcctgttt
//
