LOCUS       sequence-129619-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible gene expression in Streptomyces, C-terminal His-tag 
            encoding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Muth G
  TITLE     The pSG5-based thermosensitive vector family for genome editing and 
            gene expression in actinomycetes.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 
            10.1007/s00253-018-9334-5. Epub 2018 Sep 6.
  PUBMED    30191290
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9334-5"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-11"; volume: "102"; issue: "21"; pages: 
            "9067-9080"
COMMENT     Sequence Label: pGM1202
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..91
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(106..111,112..129,130..136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    144..160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     149..171
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     168..184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     168..184
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(362..396)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             591..608
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
       61 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      121 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
      181 tgacatgatt acgaattgta cgtaggcgtc agctacgatg ttccggggac tgctgatccg
      241 gtcagcaggt ggaagaggga ctggattcca aagttctcaa tgctgcttgc tgttcttgaa
      301 tggggggtcg ttgacgacga catggctcga ttggcgcgac aagttgctgc gattctcacc
      361 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
      421 cattactgga ccggatcggg gatctgggct gagggagccg acggcacgcg gcggctcacg
      481 gcgtggcacg cggaacgtcc gggcttgcac ctcacgtcac gtgaggaggc agcgtggacg
      541 gcgtcagaga agggagcgga catatgaaat ctcccgccct ggccggatcc catcatcatc
      601 atcatcattg actgcagcca agcttagatc cccgggtacc gagctcgaat tccccagatc
      661 taaagttttg tcgtctttcc agacgttagt aaatgaattt tctgtatgag gttttgctaa
      721 acaactttca acagtttcag cggagtgaga atagaaagga acaactaaag gaattgcgaa
      781 taataattt
//
