LOCUS       sequence-129632-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  improved luciferase reporter gene plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN
  TITLE     Generation of an improved luciferase reporter gene plasmid that 
            employs a novel mechanism for high-copy replication.
  JOURNAL   Plasmid. 2000 Sep;44(2):173-82.
  PUBMED    10964627
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "173-82"
COMMENT     Sequence Label: pXPG
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    109..243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    337..471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(602..624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     765..784
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttgtcgacc cgggtaccga gctcgagatc tgagcttgga tccagacatg
      121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      241 gttaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      301 taaagcaagt aaaacctcta caaatgtgat atggctgatc cagacatgat aagatacatt
      361 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      421 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaat
      481 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      541 aacctctaca aatgtgatat ggctgatcct gcctcgcgcg tttcggtgat gacggtgaaa
      601 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      661 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      721 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
      781 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaa
//
