LOCUS       sequence-129636-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  luciferase reporter with hGM-CSF -55 to +28 minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Bert AG, Burrows J, Osborne CS, Cockerill PN
  TITLE     Generation of an improved luciferase reporter gene plasmid that 
            employs a novel mechanism for high-copy replication.
  JOURNAL   Plasmid. 2000 Sep;44(2):173-82.
  PUBMED    10964627
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "173-82"
COMMENT     Sequence Label: pGM55
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    211..345
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    439..573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(704..726)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     867..886
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta ccaacagtac
       61 cggaatgcca agcttgtcga cagaacttta gcctttctct ctgtgtactg ggctcactgg
      121 caaaagagct cttaaataca cagaggaaat gattaatggt gacctcgcga agcttgtcga
      181 cccgggtacc gagctcgaga tctgagcttg gatccagaca tgataagata cattgatgag
      241 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      301 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caattgcatt
      361 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      421 tacaaatgtg atatggctga tccagacatg ataagataca ttgatgagtt tggacaaacc
      481 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      541 tttgtaacca ttataagctg caataaacaa gttaacaaca attgcattca ttttatgttt
      601 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtgat
      661 atggctgatc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
      721 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
      781 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
      841 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcac
//
