LOCUS       sequence-129644-       10614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10614)
  AUTHORS   He TC, Chan TA, Vogelstein B, Kinzler KW
  TITLE     PPARdelta is an APC-regulated target of nonsteroidal 
            anti-inflammatory drugs.
  JOURNAL   Cell. 1999 Oct 29. 99(3):335-45.
  PUBMED    10555149
REFERENCE   2  (bases 1 to 10614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1999 
            Oct 29. 99(3):335-45."
COMMENT     Sequence Label: pAdTrack-PPARdelta
FEATURES             Location/Qualifiers
     source          1..10614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   79..181
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        3440..3743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3744..3947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4020..4046
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    5535..5656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5742..6045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6046..6249
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6288..6297
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6294..6296,6297..6299,6300..7010)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6294..6296,6297..6299,6300..7010)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6339..6360)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6600..6619)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6947..6968
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7149..7270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     7413..7563
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   7651..7753
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      7791..8171
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7878..7897)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8088..8109
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8219..9013)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8219..9013)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8330..8349)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8940..8959
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      9845..10433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10334..10353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10587..10604
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga
       61 tccatgcatg ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg
      121 ataatgaggg ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta
      181 ggttttaggg cggagtaact tgtatgtgtt gggaattgta gttttcttaa aatgggaagt
      241 gacgtaacgt gggaaaacgg aagtgacgat ttgaggaagt tgtgggtttt ttggctttcg
      301 tttctgggcg taggttcgcg tgcggttttc tgggtgtttt ttgtggactt taaccgttac
      361 gtcatttttt agtcctatat atactcgctc tgcacttggc ccttttttac actgtgactg
      421 attgagctgg tgccgtgtcg agtggtgttt ttttaatagg ttttcttttt tactggtaag
      481 gctgactgtt atggctgccg ctgtggaagc gctgtatgtt gttctggagc gggagggtgc
      541 tattttgcct aggcaggagg gtttttcagg tgtttatgtg tttttctctc ctattaattt
      601 tgttatacct cctatggggg ctgtaatgtt gtctctacgc ctgcgggtat gtattccccc
      661 gggctatttc ggtcgctttt tagcactgac cgatgtgaat caacctgatg tgtttaccga
      721 gtcttacatt atgactccgg acatgaccga ggagctgtcg gtggtgcttt ttaatcacgg
      781 tgaccagttt ttttacggtc acgccggcat ggccgtagtc cgtcttatgc ttataagggt
      841 tgtttttcct gttgtaagac aggcttctaa tgtttaaatg tttttttgtt attttatttt
      901 gtgtttatgc agaaacccgc agacatgttt gagagaaaaa tggtgtcttt ttctgtggtg
      961 gttccggagc ttacctgcct ttatctgcat gagcatgact acgatgtgct ttcttttttg
     1021 cgcgaggctt tgcctgattt tttgagcagc accttgcatt ttatatcgcc gcccatgcaa
     1081 caagctattg aattcgttta aactccctct caagtctgta tacggggaca cggacagcct
     1141 tttcgtcacc gagcgtggac accggctcat ggaaaccaga ggtaagaaac gcatcaaaaa
     1201 gcatggggga aacctggttt ttgaccccga acggccagag ctcacctggc tcgtggaatg
     1261 cgagaccgtc tgcggggcct gcggcgcgga tgcctactcc ccggaatcgg tatttctcgc
     1321 gcccaagctc tacgccctca aaagtctgca ctgcccctcg tgcggcgcct cctccaaggg
     1381 caagctgcgc gccaagggcc acgccgcgga ggggctggac tatgacacca tggtcaaatg
     1441 ctacctggcc gacgcgcagg gcgaagaccg gcagcgcttc agcaccagca ggaccagcct
     1501 caagcgcacc ctggccagcg cgcagcccgg agcgcacccc ttcaccgtga cccagactac
     1561 gctgacgagg accctgcgcc cgtggaaaga catgaccctg gcccgtctgg acgagcaccg
     1621 actactgccg tacagcgaaa gccgccccaa cccgcgaaac gaggagatat gctggatcga
     1681 gatgccgtag agcaggtgac cgagctgtgg gaccgcctgg aactgcttgg tcaaacgctc
     1741 aaaagcatgc ctacggcgga cggtctcaaa ccgttgaaaa actttgcttc cttgcaagaa
     1801 ctgctatcgc tgggcggcga gcgccttctg gcggatttgg tcagggaaaa catgcgagtc
     1861 agggacatgc ttaacgaagt ggcccccctg ctcagggatg acggcagctg cagctctctt
     1921 aactaccagt tgcagccggt aataggtgtg atttacgggc ccaccggctg cggtaagtcg
     1981 cagctgctca ggaacctgct ttcttcccag ctgatctccc ctaccccgga aaccgttttc
     2041 ttcatcgccc cgcaggtaga catgatcccc ccatctgaac tcaaagcgtg ggaaatgcaa
     2101 atctgtgagg gtaactacgc ccctgggccg gatggaacca ttataccgca gtctggcacc
     2161 ctccgcccgc gctttgtaaa aatggcctat gacgatctca tcctggaaca caactatgac
     2221 gttagtgatc ccagaaatat cttcgcccag gccgccgccc gtgggcccat tgccatcatt
     2281 atggacgaat gcatggaaaa tcttggaggt cacaagggcg tctccaagtt cttccacgca
     2341 tttccttcta agctacatga caaatttccc aagtgcaccg gatacactgt gctggtggtt
     2401 ctgcacaaca tgaatccccg gagggatatg gctgggaaca tagccaacct aaaaatacag
     2461 tccaagatgc atctcatatc cccacgtatg cacccatccc agcttaaccg ctttgtaaac
     2521 acttacacca agggcctgcc cctggcaatc agcttgctac tgaaagacat ttttaggcac
     2581 cacgcccagc gctcctgcta cgactggatc atctacaaca ccaccccgca gcatgaagct
     2641 ctgcagtggt gctacctcca ccccagagac gggcttatgc ccatgtatct gaacatccag
     2701 agtcaccttt accacgtcct ggaaaaaata cacaggaccc tcaacgaccg agaccgctgg
     2761 tcccgggcct accgcgcgcg caaaacccct aaataaagac agcaagacac ttgcttgatc
     2821 caaatccaaa cagagtctgg ttttttattt atgttttaaa ccgcattggg aggggaggaa
     2881 gccttcaggg cagaaacctg ctggcgcaga tccaacagct gctgagaaac gacattaagt
     2941 tcccgggtca aagaatccaa ttgtgccaaa agagccgtca acttgtcatc gcgggcggat
     3001 gaacgggaag ctgcactgct tgcaagcggg ctcaggaaag caaagtcagt cacaatcccg
     3061 cgggcggtgg ctgcagcggc tgaagcggcg gcggaggctg cagtctccaa cggcgttcca
     3121 gacacggtct cgtaggtcaa ggtagtagag tttgcgggca ggacggggcg accatcaatg
     3181 ctggagccca tcacattctg acgcaccccg gcccatgggg gcatgcgcgt tgtcaaatat
     3241 gagctcacaa tgcttccatc aaacgagttg gtgctcatgg cggcggcggc tgctgcaaaa
     3301 cagatacaaa actacataag acccccacct tatatattct ttcccaccct taaccacgcc
     3361 cagatcctct agcagtgata aacgtctaat agtaatcaat tacggggtca ttagttcata
     3421 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3481 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3661 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3721 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3781 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     3841 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     3901 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     3961 gtcagatccg ctagagatct ggtaccgaaa tgggatacga tgttccagat tacgcttcgt
     4021 acgatgttcc agattacgca agcttgctag cggccgctcg aggaatggag cagccacagg
     4081 aggaagcccc tgaggtccgg gaagaggagg agaaagagga agtggcagag gcagaaggag
     4141 ccccagagct caatggggga ccacagcatg cacttccttc cagcagctac acagacctct
     4201 cccggagctc ctcgccaccc tcactgctgg accaactgca gatgggctgt gacggggcct
     4261 catgcggcag cctcaacatg gagtgccggg tgtgcgggga caaggcatcg ggcttccact
     4321 acggtgttca tgcatgtgag gggtgcaagg gcttcttccg tcgtacgatc cgcatgaagc
     4381 tggagtacga gaagtgtgag cgcagctgca agattcagaa gaagaaccgc aacaagtgcc
     4441 agtactgccg cttccagaag tgcctggcac tgggcatgtc acacaacgct atccgttttg
     4501 gtcggatgcc ggaggctgag aagaggaagc tggtggcagg gctgactgca aatgagggga
     4561 gccagtacaa cccacaggtg gccgacctga aggccttctc caagcacatc tacaatgcct
     4621 acctgaaaaa cttcaacatg accaaaaaga aggcccgcag catcctcacc ggcaaagcca
     4681 gccacacggc gccctttgtg atccacgaca tcgagacatt gtggcaggca gagaaggggc
     4741 tggtgtggaa gcagttggtg aatggcctgc ctccctacaa ggagatcagc gtgcacgtct
     4801 tctaccgctg ccagtgcacc acagtggaga ccgtgcggga gctcactgag ttcgccaaga
     4861 gcatccccag cttcagcagc ctcttcctca acgaccaggt tacccttctc aagtatggcg
     4921 tgcacgaggc catcttcgcc atgctggcct ctatcgtcaa caaggacggg ctgctggtag
     4981 ccaacggcag tggctttgtc acccgtgagt tcctgcgcag cctccgcaaa cccttcagtg
     5041 atatcattga gcctaagttt gaatttgctg tcaagttcaa cgccctggaa cttgatgaca
     5101 gtgacctggc cctattcatt gcggccatca ttctgtgtgg agaccggcca ggcctcatga
     5161 acgttccacg ggtggaggct atccaggaca ccatcctgcg tgccctcgaa ttccacctgc
     5221 aggccaacca ccctgatgcc cagtacctct tccccaagct gctgcagaag atggctgacc
     5281 tgcggcaact ggtcaccgag cacgcccaga tgatgcagcg gatcaagaag accgaaaccg
     5341 agacctcgct gcaccctctg ctccaggaga tctacaagga catgtactaa tctagataag
     5401 atatccgatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
     5461 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5521 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5581 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5641 tcatcaatgt atcttaacgc ggaggtttat cgacgatctg ctagtgatta atagtaatca
     5701 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     5761 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     5821 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     5881 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     5941 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     6001 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     6061 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     6121 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     6181 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     6241 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga
     6301 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     6361 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     6421 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     6481 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     6541 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     6601 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     6661 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     6721 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     6781 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     6841 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     6901 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     6961 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag tccggactca
     7021 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
     7081 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     7141 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7201 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7261 atgtatctta acgcgatcaa gctagcttgc tagactcgac tgactataat aataaaacgc
     7321 caactttgac ccggaacgcg gaaaacacct gagaaaaaca cctgggcgag tctccacgta
     7381 aacggtcaaa gtccccgcgg ccctagacaa atattacgcg ctatgagtaa cacaaaatta
     7441 ttcagatttc acttcctctt attcagtttt cccgcgaaaa tggccaaatc ttactcggtt
     7501 acgcccaaat ttactacaac atccgcctaa aaccgcgcga aaattgtcac ttcctgtgta
     7561 caccggcgca caccaaaaac gtcacttttg ccacatccgt cgcttacatg tgttccgcca
     7621 cacttgcaac atcacacttc cgccacacta ctacgtcacc cgccccgttc ccacgccccg
     7681 cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat
     7741 attattgatg atgttaatta agaattaatt cgatcctgaa tggcgaatgg acgcgccctg
     7801 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     7861 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     7921 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7981 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8041 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8101 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8161 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaatttt aacaaaattc
     8221 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     8281 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     8341 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     8401 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     8461 cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg gctggcgcga
     8521 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     8581 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     8641 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     8701 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     8761 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     8821 ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     8881 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     8941 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     9001 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagagctt gatcccctgc
     9061 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     9121 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     9181 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     9241 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     9301 tggctttcta cgtgaaaagg atctaggtga agatcctttt tgataatctc atggctgcag
     9361 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9421 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9481 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9541 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9601 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9661 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9721 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9781 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9841 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9901 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9961 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
    10021 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    10081 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10141 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10201 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10261 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10321 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10381 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10441 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10501 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10561 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
