LOCUS       sequence-129664-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/Streptomyces expression vector containing the T7-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Muth G
  TITLE     The pSG5-based thermosensitive vector family for genome editing and 
            gene expression in actinomycetes.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 
            10.1007/s00253-018-9334-5. Epub 2018 Sep 6.
  PUBMED    30191290
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9334-5"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-11"; volume: "102"; issue: "21"; pages: 
            "9067-9080"
COMMENT     Sequence Label: pGM202T7
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..57
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        38..56
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             88..110
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             151..183
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(187..195,196..210)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             229..246
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(438..486)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 gccgattcat taatgcagga tctcgatccc gcgaaattaa tacgactcac tatagggaga
       61 ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg
      121 cggggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg
      181 ggtcgggatc tgtacgacga tgacgataag gatccgagct cgagatccca tcatcatcat
      241 catcattgac tgcagccaag cttagatccc cgggtaccga gctcgaattc cccagatcta
      301 aagttttgtc gtctttccag acgttagtaa atgaattttc tgtatgaggt tttgctaaac
      361 aactttcaac agtttcagcg gagtgagaat agaaaggaac aactaaagga attgcgaata
      421 ataatttttt cacgttgaaa atctccaaaa aaaaaggctc caaaaggagc ctttaattgt
      481 atcggtttat cagcttgctt tcgaggtgaa tttcttaaac agcttgatac cgatagttgc
      541 gccgacaatg acaacaacca tcgcccacgc ataaccgata tattcggtcg ctgaggcttg
      601 cagggagtca aaggccgctt ttgcgggatc tcgtcgaagg cactagaggc ctatatgcgg
      661 ggatcgaccg cgcgggtccc ggacggggaa gagcggggag ctttgccaga gagcgacgac
      721 ttccccttgc gttggtgatt gccggtcagg gcagccatcc gccatcgtcg cgtagggtgt
      781 cacaccccag gaatcgcgtc actgaacaca gcagccggta ggacgaccat gactgagttg
      841 gacaccatcg caa
//
