LOCUS       sequence-129667-         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses  isochorismate-pyruvate lyase from Pseudomonas aeruginosa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Zaitseva J, Lu J, Olechoski KL, Lamb AL
  TITLE     Two crystal structures of the isochorismate pyruvate lyase from 
            Pseudomonas aeruginosa.
  JOURNAL   J Biol Chem. 2006 Nov 3;281(44):33441-9. Epub 2006 Aug 16.
  PUBMED    16914555
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-3"; volume: "281(44)"; pages: "33441-9. Epub 
            2006 Aug 16"
COMMENT     Sequence Label: pET29b- pchB WT
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             350..367
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(420..438)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      434..481
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(605..624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaagaagga gatatacata tgaaaactcc cgaagactgc accggcctgg cggacatccg
       61 cgaggccatc gaccggatcg acctggatat cgtccaggcc ctcggccgcc gcatggacta
      121 cgtcaaggcg gcgtcgcgct tcaaggccag cgaggcggcg attccggcgc ccgagcgggt
      181 cgccgcgatg ctccccgagc gcgcccgctg ggccgaggaa aacggactcg acgcgccctt
      241 cgtcgaggga ctgttcgcgc agatcatcca ctggtacatc gccgagcaga tcaagtactg
      301 gcgccagaca cggggtgccg cataataaaa gcttgcggcc gcactcgagc accaccacca
      361 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      421 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      481 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgtcag cgccctagcg
      601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      661 gctcta
//
