LOCUS       sequence-129681-         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry clone containing ER-localized Venus. Can be used to construct 
            transcriptional reporters. For use in plants and compatible with the
            MultiSite Gateway system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Siligato R, Wang X, Yadav SR, Lehesranta S, Ma G, Ursache R, Sevilem
            I, Zhang J, Gorte M, Prasad K, Wrzaczek M, Heidstra R, Murphy A, 
            Scheres B, Mahonen AP
  TITLE     MultiSite Gateway compatible cell type-specific gene inducible 
            system for plants.
  JOURNAL   Plant Physiol. 2015 Dec 7. pii: pp.01246.2015.
  PUBMED    26644504
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2015 Dec 7. pii: pp.01246.2015."
COMMENT     Sequence Label: p221z-erVenusYFP
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(165..167,168..626)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(207..228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(468..487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gcttaagctt aacaatgaag actaatcttt
      121 ttctctttct catcttttca cttctcctat cattatcctc ggccgtgagc aagggcgagg
      181 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      241 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
      301 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
      361 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      421 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      481 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      541 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      601 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      661 agatccgcca caa
//
