LOCUS       sequence-129683-        7272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Kao WH, Beaudenon SL, Talis AL, Huibregtse JM, Howley PM
  TITLE     Human papillomavirus type 16 E6 induces self-ubiquitination of the 
            E6AP ubiquitin-protein ligase.
  JOURNAL   J Virol 2000 Jul;74(14):6408-17.
  PUBMED    10864652
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol"; 
            date: "2000-07"; volume: "74(14)"; pages: "6408-17"
COMMENT     Sequence Label: p4053 HA-E6AP isoform I
FEATURES             Location/Qualifiers
     source          1..7272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..149
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     141..158
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     142..158
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        319..698
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        699..902
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     853..873
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             979..1005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3602..4224
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3801..3820)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4246..4266)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4251..4581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4432..4567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4619..4638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4620..4638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4653..4669)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4653..4669)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4666..4688)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4677..4693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4701..4707,4708..4725,4726..4731))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4746..4767
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4884..4901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5054..5642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5134..5153)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5813..6604,6605..6673))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6436..6455
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6674..6778)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6805..7233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6892..6911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7102..7123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctgatc tatacattga
      181 atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc aatattggct
      241 attggccatt gcatacgttg tatctatatc ataatatgta catttatatt ggctcatgtc
      301 caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      361 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      421 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      481 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      541 cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt gacgtcaatg
      601 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac tttcctactt
      661 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      721 ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      781 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaataacc
      841 ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      901 ctcgtttagt gaaccgtcag aattgttttt atttttaatt ttctttcaaa tacttccatc
      961 gaattcagat ccaccatgta cccatacgat gttccagatt acgctaagcg agcagctgca
     1021 aagcatctaa tagaacgcta ctaccaccag ttaactgagg gctgtggaaa tgaagcctgc
     1081 acgaatgagt tttgtgcttc ctgtccaact tttcttcgta tggataataa tgcagcagct
     1141 attaaagccc tcgagcttta taagattaat gcaaaactct gtgatcctca tccctccaag
     1201 aaaggagcaa gctcagctta ccttgagaac tcgaaaggtg cccccaacaa ctcctgctct
     1261 gagataaaaa tgaacaagaa aggcgctaga attgatttta aagatgtgac ttacttaaca
     1321 gaagagaagg tatatgaaat tcttgaatta tgtagagaaa gagaggatta ttccccttta
     1381 atccgtgtta ttggaagagt tttttctagt gctgaggcat tggtacagag cttccggaaa
     1441 gttaaacaac acaccaagga agaactgaaa tctcttcaag caaaagatga agacaaagat
     1501 gaagatgaaa aggaaaaagc tgcatgttct gctgctgcta tggaagaaga ctcagaagca
     1561 tcttcctcaa ggataggtga tagctcacag ggagacaaca atttgcaaaa attaggccct
     1621 gatgatgtgt ctgtggatat tgatgccatt agaagggtct acaccagatt gctctctaat
     1681 gaaaaaattg aaactgcctt tctcaatgca cttgtatatt tgtcacctaa cgtggaatgt
     1741 gacttgacgt atcacaatgt atactctcga gatcctaatt atctgaattt gttcattatc
     1801 gtaatggaga atagaaatct ccacagtcct gaatatctgg aaatggcttt gccattattt
     1861 tgcaaagcga tgagcaagct accccttgca gcccaaggaa aactgatcag actgtggtct
     1921 aaatacaatg cagaccagat tcggagaatg atggagacat ttcagcaact tattacttat
     1981 aaagtcataa gcaatgaatt taacagtcga aatctagtga atgatgatga tgccattgtt
     2041 gctgcttcga agtgcttgaa aatggtttac tatgcaaatg tagtgggagg ggaagtggac
     2101 acaaatcaca atgaagaaga tgatgaagag cccatccctg agtccagcga gctgacactt
     2161 caggaacttt tgggagaaga aagaagaaac aagaaaggtc ctcgagtgga ccccctggaa
     2221 actgaacttg gtgttaaaac cctggattgt cgaaaaccac ttatcccttt tgaagagttt
     2281 attaatgaac cactgaatga ggttctagaa atggataaag attatacttt tttcaaagta
     2341 gaaacagaga acaaattctc ttttatgaca tgtcccttta tattgaatgc tgtcacaaag
     2401 aatttgggat tatattatga caatagaatt cgcatgtaca gtgaacgaag aatcactgtt
     2461 ctctacagct tagttcaagg acagcagttg aatccatatt tgagactcaa agttagacgt
     2521 gaccatatca tagatgatgc acttgtccgg ctagagatga tcgctatgga aaatcctgca
     2581 gacttgaaga agcagttgta tgtggaattt gaaggagaac aaggagttga tgagggaggt
     2641 gtttccaaag aattttttca gctggttgtg gaggaaatct tcaatccaga tattggtatg
     2701 ttcacatacg atgaatctac aaaattgttt tggtttaatc catcttcttt tgaaactgag
     2761 ggtcagttta ctctgattgg catagtactg ggtctggcta tttacaataa ctgtatactg
     2821 gatgtacatt ttcccatggt tgtctacagg aagctaatgg ggaaaaaagg aacttttcgt
     2881 gacttgggag actctcaccc agttctatat cagagtttaa aagatttatt ggagtatgaa
     2941 gggaatgtgg aagatgacat gatgatcact ttccagatat cacagacaga tctttttggt
     3001 aacccaatga tgtatgatct aaaggaaaat ggtgataaaa ttccaattac aaatgaaaac
     3061 aggaaggaat ttgtcaatct ttattctgac tacattctca ataaatcagt agaaaaacag
     3121 ttcaaggctt ttcggagagg ttttcatatg gtgaccaatg aatctccctt aaagtactta
     3181 ttcagaccag aagaaattga attgcttata tgtggaagcc ggaatctaga tttccaagca
     3241 ctagaagaaa ctacagaata tgacggtggc tataccaggg actctgttct gattagggag
     3301 ttctgggaaa tcgttcattc atttacagat gaacagaaaa gactcttctt gcagtttaca
     3361 acgggcacag acagagcacc tgtgggagga ctaggaaaat taaagatgat tatagccaaa
     3421 aatggcccag acacagaaag gttacctaca tctcatactt gctttaatgt gcttttactt
     3481 ccggaatact caagcaaaga aaaacttaaa gagagattgt tgaaggccat cacgtatgcc
     3541 aaaggatttg gcatgctgta aaacaagctt gcatgcctgc aggtcgactc tagaggatcc
     3601 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc
     3661 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc
     3721 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac
     3781 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg
     3841 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt
     3901 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg
     3961 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc
     4021 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct
     4081 gattttaaaa taactatacc agcaggagga cgtccagaca cagcataggc tacctgccat
     4141 ggcccaaccg gtgggacatt tgagttgctt gcttggcact gtcctctcat gcgttgggtc
     4201 cactcagtag atgcctgttg aattgggtac gcggccagct tctgtggaat gtgtgtcagt
     4261 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4321 attagtcagc aaccaggtgt ggaaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4381 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4441 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4501 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4561 ggaggcctag gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc
     4621 ctatagtgag tcgtattaaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     4681 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     4741 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     4801 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     4861 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaggccg
     5041 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5101 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5161 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5221 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     5281 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5341 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5401 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5461 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5521 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5581 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5641 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5701 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5761 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5821 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5881 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5941 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6001 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6061 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6121 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6181 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6241 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6301 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6361 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6421 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6481 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6541 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6601 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6661 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6721 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6781 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6961 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7021 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7081 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7141 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7201 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaaca aaatattaac
     7261 gtttacaatt tc
//
