LOCUS       sequence-129689-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA protease chain with dTomato ectodomain and no linker domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: dTomato_LD 0_TEV
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(283..301)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     551..570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     745..768
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcgc catcatgtgc cgagccatct ctcttaggcg cttgctgctg ctgctgctgc
      121 agctgtcaca actcctagct gtcactcaag gggtgagcaa gggcgaggag gtcatcaaag
      181 agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg
      241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      301 agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca
      361 aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg
      421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg
      481 actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc
      541 cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc
      601 tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg
      661 gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc
      721 ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca
      781 tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgtac
//
