LOCUS       sequence-129690-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, G 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: dTomato_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(288..306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     556..575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     750..773
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgccatca tgtgccgagc catctctctt aggcgcttgc tgctgctgct
      121 gctgcagctg tcacaactcc tagctgtcac tcaaggggtg agcaagggcg aggaggtcat
      181 caaagagttc atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga
      241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      301 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg
      361 ctccaaggcg tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc
      421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac
      481 ccaggactcc tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa
      541 cttccccccc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga
      601 gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa
      661 ggacggcggc cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca
      721 actgcccggc tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta
      781 caccatc
//
