LOCUS       sequence-129694-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a targeting vector to target the endogenous Sox2 SE CPG island with 
            Snrp-Tomato-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.08"; journalName: "Cell"; date: "2015-09-24- 
            24"; volume: "163"; issue: "1"; pages: "218-29"
COMMENT     Sequence Label: SOX2se-CPG-TV
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    588..621
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc agcgcccgac
       61 cgaaaggagc gcacgacccc atgcatcgat gatatcagat ccccgggatg cagaaattga
      121 tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc atactttgtt
      181 aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct acgggggtgg
      241 gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac tattgcttta
      301 tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat taagggccag
      361 ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc attgtttttc
      421 tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca gtttcatagc
      481 ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt attgttttgc
      541 caagttctaa ttccatcaga agctggtcga gatccggaac ccttaatata acttcgtata
      601 atgtatgcta tacgaagtta ttaggtccct cgaagaggtt cactaggcgc gccggagggg
      661 gctgcattct cagacaactc tcagtggcta gcttgccctt tgacaaaact ttgctaggaa
      721 tttaccaccc ctaccccccc tccccagact ctccctttgt agctcctctc ttcccttctc
      781 tctcacttgg ctccctgctc attt
//
