LOCUS       sequence-129699-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a targeting vector, to target the endogenous Gapdh CPG island with 
            Snrp-GFP-polyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Stelzer Y, Shivalila CS, Soldner F, Markoulaki S, Jaenisch R
  TITLE     Tracing Dynamic Changes of DNA Methylation at Single-Cell 
            Resolution.
  JOURNAL   Cell. 2015 Sep 24;163(1):218-29. doi: 10.1016/j.cell.2015.08.046.
  PUBMED    26406378
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.08"; journalName: "Cell"; date: "2015-09-24- 
            24"; volume: "163"; issue: "1"; pages: "218-29"
COMMENT     Sequence Label: GAPDH-CPG-TV
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    589..622
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
       61 ccgaaaggag cgcacgaccc catgcatcga tgatatcaga tccccgggat gcagaaattg
      121 atgatctatt aaacaataaa gatgtccact aaaatggaag tttttcctgt catactttgt
      181 taagaagggt gagaacagag tacctacatt ttgaatggaa ggattggagc tacgggggtg
      241 ggggtggggt gggattagat aaatgcctgc tctttactga aggctcttta ctattgcttt
      301 atgataatgt ttcatagttg gatatcataa tttaaacaag caaaaccaaa ttaagggcca
      361 gctcattcct cccactcatg atctatagat ctatagatct ctcgtgggat cattgttttt
      421 ctcttgattc ccactttgtg gttctaagta ctgtggtttc caaatgtgtc agtttcatag
      481 cctgaagaac gagatcagca gcctctgttc cacatacact tcattctcag tattgttttg
      541 ccaagttcta attccatcag aagctggtcg agatccggaa cccttaatat aacttcgtat
      601 aatgtatgct atacgaagtt attaggtccc tcgaagaggt tcactaggcg cgcctttttg
      661 aaatgtgcac gcaccaagcg tgtgggctcc gaactgatag ggggagggga gctgagattg
      721 ccccgctcag gaggtgaccg ggtgatggtt cccaggatag gactcaggga atacagacta
      781 tgaacttaag gacgaggagt ccttggagtg tgcaccaagg acatccagga ccc
//
