LOCUS       sequence-129705-        7936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ARHGAP26.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7936)
  AUTHORS   Yao F, Kausalya JP, Sia YY, Teo AS, Lee WH, Ong AG, Zhang Z, Tan JH,
            Li G, Bertrand D, Liu X, Poh HM, Guan P, Zhu F, Pathiraja TN, 
            Ariyaratne PN, Rao J, Woo XY, Cai S, Mulawadi FH, Poh WT, Veeravalli
            L, Chan CS, Lim SS, Leong ST, Neo SC, Choi PS, Chew EG, Nagarajan N,
            Jacques PE, So JB, Ruan X, Yeoh KG, Tan P, Sung WK, Hunziker W, Ruan
            Y, Hillmer AM
  TITLE     Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces 
            Loss of Epithelial Integrity.
  JOURNAL   Cell Rep. 2015 Jul 14;12(2):272-85. doi: 
            10.1016/j.celrep.2015.06.020. Epub 2015 Jul 2.
  PUBMED    26146084
REFERENCE   2  (bases 1 to 7936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.020"; journalName: "Cell Rep"; date: 
            "2015-07-14- 14"; volume: "12"; issue: "2"; pages: "272-85"
COMMENT     Sequence Label: pMXs-Puro-ARHGAP26
FEATURES             Location/Qualifiers
     source          1..7936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1926..1935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4215..4241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4276..4296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4281..4610
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4461..4596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4620..5219
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4620..4639)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5116..5136
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5441..5910
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6040..6057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6211..6799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6291..6310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6963..7754,7755..7823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7586..7605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7824..7928)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 ccgccgccac catggggctc ccagcgctcg agttcagcga ctgctgcctc gatagtccgc
     1981 acttccgaga gacgctcaag tcgcacgaag cagagctgga caagaccaac aaattcatca
     2041 aggagctcat caaggacggg aagtcactca taagcgcgct caagaatttg tcttcagcga
     2101 agcggaagtt tgcagattcc ttaaatgaat ttaaatttca gtgcatagga gatgcagaaa
     2161 cagatgatga gatgtgtata gcaaggtctt tgcaggagtt tgccactgtc ctcaggaatc
     2221 ttgaagatga acggatacgg atgattgaga atgccagcga ggtgctcatc actcccttgg
     2281 agaagtttcg aaaggaacag atcggggctg ccaaggaagc caaaaagaag tatgacaaag
     2341 agacagaaaa gtattgtggc atcttagaaa aacacttgaa tttgtcttcc aaaaagaaag
     2401 aatctcagct tcaggaggca gacagccaag tggacctggt ccggcagcat ttctatgaag
     2461 tatccctgga atatgtcttc aaggtgcagg aagtccaaga gagaaagatg tttgagtttg
     2521 tggagcctct gctggccttc ctgcaaggac tcttcacttt ctatcaccat ggttacgaac
     2581 tggccaagga tttcggggac ttcaagacac agttaaccat tagcatacag aacacaagaa
     2641 atcgctttga aggcactaga tcagaagtgg aatcactgat gaaaaagatg aaggagaatc
     2701 cccttgagca caagaccatc agtccctaca ccatggaggg atacctctac gtgcaggaga
     2761 aacgtcactt tggaacttct tgggtgaagc actactgtac atatcaacgg gattccaaac
     2821 aaatcaccat ggtaccattt gaccaaaagt caggaggaaa agggggagaa gatgaatcag
     2881 ttatcctcaa atcctgcaca cggcggaaaa cagactccat tgagaagagg ttttgctttg
     2941 atgtggaagc agtagacagg ccaggggtta tcaccatgca agctttgtcg gaagaggacc
     3001 ggaggctctg gatggaagcc atggatggcc gggaacctgt ctacaactcg aacaaagaca
     3061 gccagagtga agggactgcg cagttggaca gcattggctt cagcataatc aggaaatgca
     3121 tccatgctgt ggaaaccaga gggatcaacg agcaagggct gtatcgaatt gtgggggtca
     3181 actccagagt gcagaagttg ctgagtgtcc tgatggaccc caagactgct tctgagacag
     3241 aaacagatat ctgtgctgaa tgggagataa agaccatcac tagtgctctg aagacctacc
     3301 taagaatgct tccaggacca ctcatgatgt accagtttca aagaagtttc atcaaagcag
     3361 caaaactgga gaaccaggag tctcgggtct ctgaaatcca cagccttgtt catcggctcc
     3421 cagagaaaaa tcggcagatg ttacagctgc tcatgaacca cttggcaaat gttgctaaca
     3481 accacaagca gaatttgatg acggtggcaa accttggtgt ggtgtttgga cccactctgc
     3541 tgaggcctca ggaagaaaca gtagcagcca tcatggacat caaatttcag aacattgtca
     3601 ttgagatcct aatagaaaac cacgaaaaga tatttaacac cgtgcccgat atgcctctca
     3661 ccaatgccca gctgcacctg tctcggaaga agagcagtga ctccaagccc ccgtcctgca
     3721 gcgagaggcc cctgacgctc ttccacaccg ttcagtcaac agagaaacag gaacaaagga
     3781 acagcatcat caactccagt ttggaatctg tctcatcaaa tccaaacagc atccttaatt
     3841 ccagcagcag cttacagccc aacatgaact ccagtgaccc agacctggct gtggtcaaac
     3901 ccacccggcc caactcactc cccccgaatc caagcccaac ttcacccctc tcgccatctt
     3961 ggcccatgtt ctcggcgcca tccagcccta tgcccacctc atccacgtcc agcgactcat
     4021 cccccgtcag cacaccgttc cggaaggcaa aagccttgta tgcctgcaaa gctgaacatg
     4081 actcagaact ttcgttcaca gcaggcacgg tcttcgataa tgttcaccca tctcaggagc
     4141 ctggctggtt ggaggggact ctgaacggaa agactggcct catccctgag aattacgtgg
     4201 agttcctcct cgagtaccca tacgatgttc cagattacgc ttgatgagcg gccgccagca
     4261 cagtggtcga cctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     4321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     4381 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     4441 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4561 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttacca
     4621 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4681 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4741 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4801 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4861 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4921 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4981 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5041 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5101 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5161 acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgac
     5221 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgacccatc gtcgacgata
     5281 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     5341 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     5401 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     5461 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     5521 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5581 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     5641 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     5701 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     5761 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     5821 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     5881 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     5941 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     6001 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6061 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6121 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6181 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6241 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6301 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6361 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6421 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6481 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6541 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6601 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6661 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6721 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6781 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6841 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6901 gatccttttg cggccggccg caaatcaatc taaagtatat atgagtaaac ttggtctgac
     6961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     7021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7921 gttccgcgca catttc
//
