LOCUS       sequence-129708-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli/Streptomyces expression vector containing the T7-promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Muth G
  TITLE     The pSG5-based thermosensitive vector family for genome editing and 
            gene expression in actinomycetes.
  JOURNAL   Appl Microbiol Biotechnol. 2018 Nov;102(21):9067-9080. doi: 
            10.1007/s00253-018-9334-5. Epub 2018 Sep 6.
  PUBMED    30191290
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-018-9334-5"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2018-11"; volume: "102"; issue: "21"; pages: 
            "9067-9080"
COMMENT     Sequence Label: pGM202T7
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(388..407)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
       61 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggggtgg gcgaagaact
      121 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
      181 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg
      241 tcgcttggtc ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
      301 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
      361 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
      421 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
      481 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
      541 ccgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
      601 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
      661 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
      721 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
      781 cacttcgcc
//
