LOCUS       sequence-129709-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses  isochorismate-pyruvate lyase from Pseudomonas aeruginosa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Zaitseva J, Lu J, Olechoski KL, Lamb AL
  TITLE     Two crystal structures of the isochorismate pyruvate lyase from 
            Pseudomonas aeruginosa.
  JOURNAL   J Biol Chem. 2006 Nov 3;281(44):33441-9. Epub 2006 Aug 16.
  PUBMED    16914555
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-3"; volume: "281(44)"; pages: "33441-9. Epub 
            2006 Aug 16"
COMMENT     Sequence Label: pET29b- pchB WT
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             370..392
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    407..431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(431..450)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(432..450)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(538..557)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag cttttattat
       61 gcggcacccc gtgtctggcg ccagtacttg atctgctcgg cgatgtacca gtggatgatc
      121 tgcgcgaaca gtccctcgac gaagggcgcg tcgagtccgt tttcctcggc ccagcgggcg
      181 cgctcgggga gcatcgcggc gacccgctcg ggcgccggaa tcgccgcctc gctggccttg
      241 aagcgcgacg ccgccttgac gtagtccatg cggcggccga gggcctggac gatatccagg
      301 tcgatccggt cgatggcctc gcggatgtcc gccaggccgg tgcagtcttc gggagttttc
      361 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
      421 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatcg atctcgatcc
      481 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      541 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      601 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      661 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      721 tcctaatgca ggagtcgcat aagggagagc gtcgagatcc cggacaccat c
//
