LOCUS       sequence-129710-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tn7-based lux reporter for promoter cloning in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Glassing A, Lewis TA
  TITLE     An improved Tn7-lux reporter for broad host range, 
            chromosomally-integrated promoter fusions in Gram-negative bacteria.
  JOURNAL   J Microbiol Methods. 2015 Sep 1;118:75-77. doi: 
            10.1016/j.mimet.2015.08.016.
  PUBMED    26341612
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Microbiol
            Methods."; date: "2015-09-1"; volume: "118:75-77. doi"; pages: " 
            10.1016/j.mimet.2015.08.016"
COMMENT     Sequence Label: pAG4
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(185..190,191..207,208..213))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    254..301
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
       61 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
      121 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
      181 ttacagttta cgaaccgaac aggcttatgt caattcgatc cattgctgtt gacaaaggga
      241 atcaggggat cttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt
      301 cagagcgctt ttgaagctaa ttcgatcatg catgagctca ctagtggatc ccccgggctg
      361 caggaattcg atcatggatg gcaaatatga ctaaaaaaat ttcattcatt attaacggcc
      421 aggttgaaat ctttcccgaa agtgatgatt tagtgcaatc cattaatttt ggtgataata
      481 gtgtttacct gccaatattg aatgactctc atgtaaaaaa cattattgat tgtaatggaa
      541 ataacgaatt acggttgcat aacattgtca attttctcta tacggtaggg caaagatgga
      601 aaaatgaaga atactcaaga cgcaggacat acattcgtga cttaaaaaaa tatatgggat
      661 attcagaaga aatggctaag ctagaggcca attggatatc tatgatttta tgttctaaag
      721 gcggccttta tgatgttgt
//
