LOCUS       sequence-129740-        6074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD
            sequence-MCSII-sspH2 signal peptide, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6074)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 6074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 56
FEATURES             Location/Qualifiers
     source          1..6074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             999..1021
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1535..1554)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1536..1554)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1564..1581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1564..1580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1573..1595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1722..2177
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1809..1828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2019..2040
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2203..2307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2308..2376,2377..3168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2526..2545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4191..5141)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5189..5411
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcccaattcg gttcacccgg gaaataattt tgtttaactt taagaaggag
     1021 atatacatat gccctttcat attggaagcg gatgtcttcc cgccaccatc agtaatcgcc
     1081 gcatttatcg tattgcctgg tctgataccc cccctgaaat gagttcctgg gaaaaaatga
     1141 aggaattttt ttgctcaacg caccagactg aagcgctgga gtgcatctgg acgatttgtc
     1201 acccgccggc cggaacgacg cgggaggatg tgatcaacag atttgaactg ctcaggacgc
     1261 tcgcgtatgc cggatgggag gaaagcattc attccggcca gcacggggaa aattacttct
     1321 gtattctgga tgaagacagt caggagatat tgtcagtcac ccttgatgat gccgggaact
     1381 ataccgtaaa ttgccagggg tacagtgaaa cacatcgcct caccctggac acagcacagg
     1441 gtgaggaggg cacaggacac gcggaagggg catccgggct gcagatatcc atggtaccga
     1501 gctcggatcc gtcgacaagc ttatcgccaa ttcgccctat agtgagtcgt attacgcgcg
     1561 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     1621 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     1681 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     1741 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     1801 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     1861 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     1921 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     1981 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     2041 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     2101 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     2161 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2281 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2341 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2401 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2461 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2521 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2581 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2641 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2701 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2761 aacatggggg atcatgtaac tcgccttgat cgctgggaac cggagctgaa tgaagccata
     2821 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     2881 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     2941 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3001 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3061 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3121 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3181 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3241 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3301 tgagcgtcag atctctgttc gggggttcac tgacgctgcg tgactacgat ggtcaggttg
     3361 cggaggctat ggccctggta cgagcgctga acaaaatgac gaaagcaggt atgcctgaaa
     3421 gcgtgcgtat tgcctgaaaa cacaacccgc tacgggggag acttacccga aatctgattt
     3481 attcaacaaa gccactttca gtatggattt gggtgttacg ggggggggtt ggtgcttaaa
     3541 atcgcgctga ggatttctga tggtgttaag cgggcggttt tgagatgtaa actcgcccgt
     3601 ttaacataat ggatcttgcg cgcaccgccc gaacaccact cgccacaaaa aaccgccgga
     3661 acgtccaaaa gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt
     3721 tatcagaatc gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag
     3781 aattgccatg attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt
     3841 gattcgataa gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca
     3901 agttgtctca ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt
     3961 ctattaggtg ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct
     4021 ttgaatgcac caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca
     4081 tctgtgcata tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     4141 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     4201 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     4261 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     4321 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     4381 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     4441 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     4501 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     4561 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     4621 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     4681 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     4741 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     4801 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     4861 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     4921 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     4981 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     5041 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     5101 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     5161 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     5221 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     5281 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     5341 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     5401 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     5461 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     5521 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     5581 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     5641 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     5701 cacgggcttc tcagggcgtt ttatggcggg tctgcggagt ggtgaatccg ttagcgaggt
     5761 gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg
     5821 gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc
     5881 cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag
     5941 agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
     6001 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
     6061 catccagcct cgcg
//
