LOCUS       sequence-129741-        6184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF
            attenuator-T7 SD sequence-MCSII- sspH2 signal peptide, M13 replicat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 6184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 62
FEATURES             Location/Qualifiers
     source          1..6184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     RBS             1109..1131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1645..1664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1646..1664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1674..1691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1674..1690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1683..1705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1832..2287
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1919..1938)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2129..2150
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2313..2417
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2418..2486,2487..3278)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2636..2655)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4301..5251)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5299..5521
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcgagtgaat aaaaggtttt gggcagcgcg ccaatggcgg cgcgtatgtc
     1021 cagggataaa ggcgtccagc ggtgcgtaag caccgccggg cgcttttttt ttgcgcgaat
     1081 tcccaattcg gttcacccgg gaaataattt tgtttaactt taagaaggag atatacatat
     1141 gccctttcat attggaagcg gatgtcttcc cgccaccatc agtaatcgcc gcatttatcg
     1201 tattgcctgg tctgataccc cccctgaaat gagttcctgg gaaaaaatga aggaattttt
     1261 ttgctcaacg caccagactg aagcgctgga gtgcatctgg acgatttgtc acccgccggc
     1321 cggaacgacg cgggaggatg tgatcaacag atttgaactg ctcaggacgc tcgcgtatgc
     1381 cggatgggag gaaagcattc attccggcca gcacggggaa aattacttct gtattctgga
     1441 tgaagacagt caggagatat tgtcagtcac ccttgatgat gccgggaact ataccgtaaa
     1501 ttgccagggg tacagtgaaa cacatcgcct caccctggac acagcacagg gtgaggaggg
     1561 cacaggacac gcggaagggg catccgggct gcagatatcc atggtaccga gctcggatcc
     1621 gtcgacaagc ttatcgccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     1681 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1741 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1801 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     1861 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1921 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1981 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2041 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2101 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2161 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2221 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     2281 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2341 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2401 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2461 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2521 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2581 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2641 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2701 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2761 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2821 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2881 atcatgtaac tcgccttgat cgctgggaac cggagctgaa tgaagccata ccaaacgacg
     2941 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     3001 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     3061 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3121 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3181 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3241 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3421 atctctgttc gggggttcac tgacgctgcg tgactacgat ggtcaggttg cggaggctat
     3481 ggccctggta cgagcgctga acaaaatgac gaaagcaggt atgcctgaaa gcgtgcgtat
     3541 tgcctgaaaa cacaacccgc tacgggggag acttacccga aatctgattt attcaacaaa
     3601 gccactttca gtatggattt gggtgttacg ggggggggtt ggtgcttaaa atcgcgctga
     3661 ggatttctga tggtgttaag cgggcggttt tgagatgtaa actcgcccgt ttaacataat
     3721 ggatcttgcg cgcaccgccc gaacaccact cgccacaaaa aaccgccgga acgtccaaaa
     3781 gtacgggttt tgctgcccgc aaacgggctg ttctggtgtt gctagtttgt tatcagaatc
     3841 gcagatccgg cttcagccgg tttgccggct gaaagcgcta tttcttccag aattgccatg
     3901 attttttccc cacgggaggc gtcactggct cccgtgttgt cggcagcttt gattcgataa
     3961 gcagcatcgc ctgtttcagg ctgtctatgt gtgactgttg agctgtaaca agttgtctca
     4021 ggtgttcaat ttcatgttct agttgctttg ttttactggt ttcacctgtt ctattaggtg
     4081 ttacatgctg ttcatctgtt acattgtcga tctgttcatg gtgaacagct ttgaatgcac
     4141 caaaaactcg taaaagctct gatgtatcta tcttttttac accgttttca tctgtgcata
     4201 tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag
     4261 tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag
     4321 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
     4381 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
     4441 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg
     4501 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
     4561 cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc
     4621 gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca
     4681 aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact
     4741 taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt
     4801 ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca
     4861 gattatattt tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa
     4921 ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc
     4981 ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag
     5041 ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag
     5101 tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca
     5161 cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt
     5221 catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat
     5281 cactatacca attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg
     5341 tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct
     5401 ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt
     5461 atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact
     5521 cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc
     5581 tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat
     5641 cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac
     5701 attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc
     5761 cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc
     5821 tcagggcgtt ttatggcggg tctgcggagt ggtgaatccg ttagcgaggt gccgccggct
     5881 tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca
     5941 aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca
     6001 taaatcgccg tgacgatcag cggtccaatg atcgaagtta ggctggtaag agccgcgagc
     6061 gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac
     6121 gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct
     6181 cgcg
//
